When recombineering bacterial artificial chromosomes (BACs), it is common practice to design the ends of the donor molecule with 50 bp of homology specifying its insertion site. We demonstrate that desired recombinants can be produced using intermolecular homologies as short as 15 bp. Although the use of shorter donor end regions decreases total recombinants by several fold, the frequency of re...

A 57-year-old man was diagnosed with AL amyloidosis after presenting with nephrotic syndrome. He had been well until 6 months earlier, when he noticed intermittent ankle swelling. He sought medical attention approximately 2 months later because of increased swelling of his legs and progressive fatigue. At that time, his urinary protein excretion was 7.4 g/day. Serum creatinine was 0.9 mg/dL and...

A low molecular weight protein (approximately 25,000 D) exhibiting a yellow fluorescence emission peaking at approximately 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (lambda max = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the ...

The Red recombination system of bacteriophage lambda is widely used for genetic engineering because of its ability to promote recombination between bacterial chromosomes or plasmids and linear DNA species introduced by electroporation. The process is known to be intimately tied to replication, but the cellular functions which participate with Red in this process are largely unknown. Here two su...

which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. A 53-year-old man presented with a history of weight loss of 10 kg over 3 months. A peripheral blood count indicated a leukocyte count of 49.0×10 3 /L and 80% segmented neutrophils. The neutrophils showed toxic changes and appeared in hypersegmented forms. ...

‎in this paper‎, ‎we study a class of boundary value problem‎ ‎involving the p-laplacian oprator and singular nonlinearities‎. ‎we‎ ‎analyze the existence a critical parameter $lambda^{ast}$ such‎ ‎that the problem has least one solution for‎ ‎$lambdain(0,lambda^{ast})$ and no solution for‎ ‎$lambda>lambda^{ast}.$ we find lower bounds of critical‎ ‎parameter $lambda^{ast}$‎. ‎we use the method ...