To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XylC from Pseudomonas putida), the lacZ-based host responded to t...

Striking similarities continue to emerge between the mammalian and Drosophila JAK/STAT signaling pathway. However, until now there has not been the ability to monitor global pathway activity during development. We have generated a transgenic animal with a JAK/STAT responsive reporter gene that can be used to monitor pathway activation in whole Drosophila embryos. Expression of the lacZ reporter...

Objective: Ty2-917 is a low copy retrotransposon found in the Saccharomyces cerevisiae genome. It has structural similarities to metazoan retroviruses terms of genome organization and propagation mechanisms host cells. The objective this study analyze effects autophagy signaling on transcriptional regulation Ty2 yeast Materials Methods: Ty2-LacZ gene fusions YEp vectors have been used as report...

Two recombinant retroviral systems are described that can be used to analyze antiretroviral drug activity and HIV breakthrough (replication in the presence of the drug). The first system utilizes a recombinant HIV encoding beta-galactosidase as a reporter gene (HIV-LacZ). The defective HIV-LacZ virus is produced in COS cells after co-transfection of a plasmid encoding the HIV-LacZ genome with a...

The hepatic nuclear factor 3gamma (Hnf3g) is a member of the winged helix gene family of transcription factors and is thought to be involved in anterior-posterior regionalization of the primitive gut. In this study, cis-regulatory elements essential for the expression of Hnf3g in vivo have been characterized. To this end, a 170 kb yeast artificial chromosome (YAC) carrying the entire Hnf3g locu...

Because of the extreme diversity in immunoglobulin genes, tolerance mechanisms are necessary to ensure that B cells do not respond to self-antigens. One such tolerance mechanism is called receptor editing. If the B cell receptor (BCR) on an immature B cell recognizes self-antigen, it is down-regulated from the cell surface, and light chain gene rearrangement continues in an attempt to edit the ...

1 The gene encoding mouse cyclin A1, Ccna1, is expressed at highest levels in late 2 pachytene-diplotene spermatocytes, where it is required for meiotic cell division. To 3 begin to understand the mechanisms responsible for its highly restricted pattern of 4 expression, transgenic mouse lines carrying constructs consisting of the cyclin A1 5 regulatory region fused with the reporter gene lacZ w...

PURPOSE We previously demonstrated that 350 bp of the human rod cGMP phosphodiesterase beta-subunit (beta-PDE) gene promoter are sufficient to direct high levels of gene expression in human Y-79 retinoblastoma cells in vitro. In this study the cell specificity and expression pattern conferred by the short beta-PDE 5' flanking sequence in vivo were examined. METHODS A construct containing the ...

DNA methylation is one of the mechanism for regulating gene expression. Transfected cells in tissue culture are a mosaic of expression of the exogenous gene. 5’ azacytidine (Aza C), inhibiting nuclear DNA methyltransferase, causes an increase of expression of E.coli a-galactosidase (LacZ) in transfected CHO cells. Murine muscle cell lines G8 and C2C12 were transfected by biolistic technique wit...