Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Leloir pathway enzymes. These were organized into an operon in the order galKTE, ...

Dairy and fish isolates of Lactococcus garvieae were tested for their ability to utilize lactose and to grow in milk. Fish isolates were unable to assimilate lactose, but unexpectedly, they possessed the ability to grow in milk. Genetic studies, carried out constructing different vectorette libraries, provided evidence that in fish isolates, no genes involved in lactose utilization were present...

Introduction. The main criterion for the state of water in food is indicator "water activity" (Aw), which affects their quality and shelf life. Problem. At present, it advisable to propose such changes recipe technology canned milk, would reduce rate increase osmotic pressure. aim work scientific substantiation factors milk preservation. Methods. Objects research – low-fat condensed low-lactose...

The present study demonstrates the immobilization of Aspergillus oryzae β-galactosidase on cellulose acetate-polymethylmethacrylate (CA-PMMA) membrane and its application in hydrolyzing lactose in dairy industries. The effect of physical and chemical denaturants like pH, temperature, product inhibition by galactose, storage stability, and reuse number of the enzyme immobilized on CA-PMMA membra...

Sucrose, unlike the other common disaccharides, maltose and lactose, has been reported to be absorbed poorly from jejunum in man. Using a single-lumen tube technique and giving a carbohydrate-protein-fat meal with a single disaccharide as the source of carbohydrate, Dahlqvist and Borgstr6m (1) reported efficient absorption of lactose and maltose from human jejunum but minimal absorption of sucr...

The studies reported in this paper were directed to an explanation of the puzzling phenomenon that the non-lactose-fermenting Escherichia coli-mutabile contains lactase (Deere, Dulaney and Michelson, 1939). Earlier experiments led us to believe that the antiseptics employed "activated" the lactase which was present, but inactive, in living growing cultures of the non-lactose-fermenting (white) ...

Regulation of the beta-galactoside transport system in response to growth substrates in the extremely thermophilic anaerobic bacterium Thermotoga neapolitana was studied with the nonmetabolizable analog methyl-beta-D-thiogalactopyranoside (TMG) as the transport substrate. T. neapolitana cells grown on galactose or lactose accumulated TMG against a concentration gradient in an intracellular free...