By Biuu’ ui n A. COOPER AND Louis LOWENSTEIN T HE DETERMINATION of folate activity in serum with Lactobacillus casei allows recognition of patients with folate deficien y.13 It has been observed that much of the L. casei-active folate in human serum consists of N5-methyl tetrahydropteroyl monoglutamate,4 and that the concentration of folate activity in serum is increased in some patients with p...

The antibacterial activity of Lactobacillus casei IMV B-7280, Lact. acidophilus IMV B-7279, Bifidobacterium longum VK1, and B. bifidum VK2 strains or their various compositions in relation to Staphylococcus aureus in vitro and on models of experimental intravaginal staphylococcosis of mice was determined. It was found that under the influence of these strains and their various compositions, the...

Lactobacillus rhamnosus LOCK900 fulfills the criteria required for probiotic strains. In this study, we report a whole-genome sequence of this isolate and compare it with other L. rhamnosus complete genome sequences already published.

Maintenance of the immunological tolerance and homeostasis in the gut is associated with the composition of the intestinal microbiota. We here report that cultivation of Lactobacillus casei ATCC 334 in the presence of human intestinal epithelial cells promotes functional changes in bacteria. In particular, the interaction enhanced the immunosuppressive phenotype of L. casei as demonstrated by t...

Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at -18 degrees C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microorganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay...

The Lactobacillus casei response to folate is normally estimated by measuring the culture turbidity after 16 to 20 hours' incubation. Streeter and O'Neill (1969) found that cultures containing serum grew more rapidly than similar cultures containing folic acid (PteGlu) unless grown into the stationary phase (44 hours). Others, however, have reported comparable results from 20and 44-hour assays ...

Agitation of broth cultures of Lactobacillus casei retarded cellular dry weight accumulation but enhanced production of both diacetyl and acetoin. Addition of pyruvate overcame this retardation, but addition of sulfhydryl-protecting reagents did not. Both pyruvate and citrate enhanced accumulated dry weight of L. casei incubated without agitation, but only pyruvate increased diacetyl accumulati...

REID, V. E., AKD FRIEDKIN, M. 1973. Plasnzodizlfn bcrghci: Folic Acid Levels in Mouse Erythrocytes. Expcri~~cntal Purasifology 33, 424-428 (1973). Folate levels of parasitized whole blood increased over fourfold above noninfected controls based on microbiological assays with Lactobacillus casei. Most of this increase is attributed to oxidized forms of folate (mono, di, and triglutamates) and/or...

Two strains were selected--the lactoso-negative yeast Rhodotorula rubra GED2 and the homofermentative Lactobacillus casei subsp. casei Ha1 for co-cultivation in cheese whey ultrafiltrate (WU) and active synthesis of carotenoids. Under conditions of intensive aeration (1.0 l/l min, 220 rpm), a temperature of 30 degrees C, WU with 55.0 g lactose/l, initial pH = 5.5, the carotenoid content in the ...

Background Allergies are becoming a serious health burden in developed countries. Increasing numbers of clinical trials and animal experiments show that probiotics are new promising tools in allergy prophylaxis. In the present work we compared anti-allergic properties of the recently described probiotic mixture of Lactobacillus casei LOCK0900, L. casei LOCK0908 and L. paracasei LOCK0919 and L. ...