Background/Aims Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. Materials and Methods HT-29 and Caco...

Bile salt hydrolase (BSH), a widely distributed function of the gut microbiota, has a profound impact on host lipid metabolism and energy harvest. Recent studies suggest that BSH inhibitors are promising alternatives to antibiotic growth promoters (AGP) for enhanced animal growth performance and food safety. Using a high-purity BSH from Lactobacillus salivarius strain, we have identified a pane...

Two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. Annotation of the complete genome sequence of the probiotic bacterium Lactobacillus acidophilus NCFM revealed nine two-component regulatory systems. In this study, the histidine protein kinase of a two-component regulatory system (LBA1524HPK-LBA1525RR), similar to the acid-related syste...

OBJECTIVES Recent studies have reported dysregulated expression of matrix metalloproteinases (MMPs), especially MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1, -2 (TIMP-1, TIMP-2), and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in activated macrophages of patients with inflammatory diseases. Therefore, MMP-2, MMP-9, and their regulators may represent a new target for ...

Crystalline arrays of proteinaceous subunits forming surface layers (S-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. The surface layer protein of Lactobacillus acidophilus ATCC4356 is composed of a single species of protein of apparent molecular weight of 43-46 KDa. Considering the Lactobacillus acidophilus ATCC4356 having the ...

Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer of the cell envelope. Many additional proteins that are associated with or embedded within the S-layer have been identified in Lactobacillus acidophilus NCFM, an S-layer-forming bacterium that is widely used in fermented dairy prod...

Probiotic ice cream was made by fermenting a standard ice cream mix with Lactobacillus acidophilus and Bifidobacterium bifidum cultures and then freezing the mix in a batch freezer. Survival of the L. acidophilus and B. bifidum, as well as beta-galactosidase activity, was monitored during 17 wk of frozen storage at -29 degrees C. After freezing of the fermented mix, bacterial counts were 1.5 x ...

conclusions the results showed that the effect of l. acidophilus is time-dependent. materials and methods antioxidant biomarkers, such as total antioxidant power (tap), measured with the frap (ferric-reducing ability of plasma) method, were evaluated at 24, 48, and 72 hours. results the results showed that extracts and bacteria of l. acidophilus were able to significantly increase tap after 24 ...

Extensive information has been accumulated concerning the organic nutrient requirements of lactic acid bacteria. Studies of the inorganic requirements of these organisms have been pursued less vigorously. Several investigations (Woolley, 1941; McLeod and Snell, 1947; Rogosa, 1944) have been undertaken to determine the essentiality of Mn++, Mg++, K+, Fe++, and PO-for Lactobacillus caswi, strain ...

The sulfur nutrition of microorganisms has been studied by many investigators. Early workers were interested essentially in sulfur compounds used by organisms as sulfur sources. Recent efforts have been directed toward the determination of mechanisms involved in the utilization of sulfur compounds. Results of investigations by Horowitz (1947), Lampen et al. (1947), Teas et al. (1948), Binkley a...