Rhizopus oryzae is used for industrial production of lactic acid, yet little is known about the genetics of this fungus. In this study I cloned two genes, IdhA and IdhB, which code for NAD+-dependent L-lactate dehydrogenases (LDH) (EC 1.1.1.27), from a lactic acid-producing strain of R. oryzae. These genes are similar to each other and exhibit more than 90% nucleotide sequence identity and they...

The production of value-added chemicals from the bioconversion of lignocellulose biomass has been considered a promising venture. In this study, microwave, alkali-pretreated empty fruit bunch (EFB) was used as the substrate, utilizing pelletized filamentous Rhizopus oryzae NRRL 395 and cellulolytic enzymes for lactic acid production in a fed-batch simultaneous saccharification and fermentation ...

Under aerobic growth conditions Lactobacillus plantarum produced acetic acid in addition to lactic acid. It was found that lactic acid was predominantly produced at first, and then when the carbohydrate was nearly exhausted, lactic acid was metabolized further to acetic acid. The most likely enzyme involved in the aerobic metabolism of L. plantarum is pyruvate oxidase. Its activity is enhanced ...

Several strains belonging to the genus Bifidobacterium were tested to determine their abilities to produce succinic acid. Bifidobacterium longum strain BB536 and Bifidobacterium animalis subsp. lactis strain Bb 12 were kinetically analyzed in detail using in vitro fermentations to obtain more insight into the metabolism and production of succinic acid by bifidobacteria. Changes in end product f...

Most of the Gram-positive bacteria, including lactic acid bacteria, produce exopolysaccharides (EPS). EPS are either excreted into the growth medium (slime) or attached to the bacterial cell wall (capsules). They are long-chain, high molecular carbohydrate polymers. In dairy industry, EPS producing cultures provide the fermentation produced with viscosity, stability and water binding functions....

BACKGROUND Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass. RESULTS A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inh...

D-lactic acidosis or D-lactate encephalopathy is a rare condition that occurs primarily in individuals who have a history of short bowel syndrome. The unabsorbed carbohydrates act as a substrate for colonic bacteria to form D-lactic acid among other organic acids. The acidic pH generated as a result of D-lactate production further propagates production of D-lactic acid, hence giving rise to a v...

A lactic-acid-producing strain was isolated from the Brazilian Cerrado soil (Brazilian savanna). Glycerol, a byproduct of biodiesel industry, can be converted into various chemical intermediates industrial value by biotechnological routes. Klebsiella pneumoniae metabolize glycerol in environments with or without oxygen and bioconvert it several chemicals high value-added, such as lactic acid, 3...

Acid tolerance of the lactic acid bacterium (LAB) is crucially important for production free as a chemical monomer by simplified purification steps. This study conducts both metabolic modification and adaptive evolution approaches on increasing an engineered Pediococcus acidilactici strain. The overexpression genes encoding lactate dehydrogenase, recombinase, chaperone, glutathione ATPase did n...