BACKGROUND Serum lactate dehydrogenase is a non-specific marker for lymphoma whose prognostic significance is well established for both indolent and aggressive lymphomas at the time of diagnosis. The performance characteristics of this enzyme in predicting relapse in patients with diffuse large B-cell lymphoma has not been well studied. METHODS This study compared serum lactate dehydrogenase ...

Bisphenol F (BPF) has been used frequently in the plastics industry and production of daily consumer products as an alternative to bisphenol A (BPA). It was aimed herein determine cytotoxic effects BPF on hepatocytes isolated from liver rainbow trout (Oncorhyncus mykiss) using lactate dehydrogenase (LDH) assay antioxidant defence system indicators. The cultured were exposed seven concentrations...

Recently, an in vitro enzymatic cascade was constructed to transform glycerol into the high-value platform chemical pyruvate. However, low activity of dihydroxy acid dehydratase from Sulfolobus solfataricus (SsDHAD) limited efficiency. In this study, reduction pyruvate catalyzed by d-lactate dehydrogenase Pseudomonas aeruginosa PAO1 used assay activities dehydratases. Dihydroxy Paralcaligenes u...

UNLABELLED L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD+-dependent L-lactate dehydrogenase. Naturally, Escherichia coli does not produce L-lactate in noticeable amounts, but can catabolize it via a dehydrogenation reaction mediated by an FMN-dependent L-lactate dehydrogenase...

The tinetics of NADH, 3-thionicotinamide adenine dinucleotide (TNAD), and oxamate binding to the H4 isoenzyme of lactate dehydrogenase from the pig has been investigated by temperature jump techniques. The dissociation rate constant for TNAD is considerably larger than for NADH, whereas the recombination rate constant is smaller for the oxidized coenzyme than for the reduced molecule. The kinet...