Among GRAS molecules, α-amino acids have been extensively used to produce molecular salts and cocrystals of APIs thanks their nontoxicity, ready availability, cheapness, zwitterionic nature. Here we report on the use both anhydrous hydrated l-proline (Pro Pro·H2O, respectively) with methyl gallate (MG) selectively obtain by mechanochemical methods cocrystals: MG·Pro2 MG2·Pro2·H2O, respectively....

This study has demonstrated the existence of an L-proline-dependent (Na independent) proton flux at the apical membrane level of the eel intestinal absorbing cells. Using isolated eel enterocytes and the pH-sensitive fluorescent dye 2', 7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester (BCECF), it was shown that a 20 mM concentration of the imino acid L-proline in the ex...

ornithine is a non-proteinogenic amino acid, which plays an essential role in the metabolism of plants. regard to the chirality of the molecule, physiological response of the plant cells to its two enantiomers have not been widely investigated yet. in the present study, suspension-cultured tobacco cells were treated with 1 mm of d- and lenantiomers of ornithine in normal conditions as well as u...

The Front Cover represents the discovery of a novel path during scientist′s journey towards biocatalytic incorporation chlorine into L-proline. In their Communication, A. Papadopoulou et al describe remodeling an Fe/αKG hydroxylase halogenase utilizing tools protein engineering. By employing several rounds directed evolution, remodeled variant with 98-fold improved apparent kcat/Km was generate...

The direct three component modified Mannich reaction via condensation of aldehydes, 2-naphthol or 2,7-naphthalendiol and piperidine to generate Betti bases has been carried out over L-proline (20 mol%) with high efficiency under solvent free conditions at 70 OC. Also, the reaction of aromatic aldehyde and 4-hydroxycoumarin in the presence of L-proline (20 mol%) in the ethanol under reflux condi...

The transport of L-proline was studied in brush-border membrane vesicles isolated from the kidneys of newborn rats. In contrast with the rapid initial uptake with an 'overshoot' observed in adult vesicles, uptake by the newborn vesicle was slow, showed no 'overshoot', and proline continued to accumulate at a time when the adult vesicle had already equilibrated. L-Proline transport in the newbor...

Fractions of poly-L-proline Form II ranging in molecular weight from 1,600 to 21,000 were compared in their ability to serve as competitive inhibitors for the synthesis of hydroxyproline by protocollagen proline hydroxylase. The degree of inhibition of the enzyme increased with the size of the polymer, and the Ki values decreased progressively with molecular weight. The results indicated that p...

To evaluate the physiological responses of chrysanthemum to silicon spray in non-stress conditions, this study was performed in two separate experiments (at 4 to 5 and 8 to 12-leaf stages) as a factorial randomized completely design with two factors: silicon source (in two levels: sodium silicate and calcium silicate) and the concentration of silicate at five levels (0, 50, 100, 150 and 200 mg ...