The peripheral plasma content of the pulmonary metabolite, 13,14-dihydro-15-keto-prostaglandin E2, reflects prostaglandin E2 (PGE2) biosynthesis and disposition in vivo more reliably than the peripheral plasma content of PGE2 itself. However, the chemical instability of 13,14-dihydro-15-keto-PGE2 and the multiple fates of its degradation products hinder the development of quantitative assays fo...

We studied the individual and combined effects of extracellular acidosis and increases in extracellular potassium on action potential characteristics and conduction in order to gain a better understanding of the effects of acute ischemia. At each level of potassium between 2.7 and 17 mm, acidosis induced by increasing Pco2 (respiratory acidosis) and by decreasing HCO3- (metabolic acidosis) decr...

Concentrations of prostaglandins E, F, and 6-keto-prostaglandin F1 alpha were estimated in central venous blood and amniotic fluid in 21 women with eclampsia and 16 healthy pregnant controls. Central venous blood concentrations of 6-keto-prostaglandin F1 alpha and prostaglandin E were significantly lower in patients than controls before delivery and remained reduced for at least 48 hours after ...

Background: The aim of this study was to evaluate the efficacy of arterial blood gases (ABGs) in determining the type of intoxication. Methods: Medical charts of all patients who had referred to the toxicology ward of Baharloo Hospital between January 2009 and December 2009 were evaluated. After excluding the patients with multi-drug toxicity, data, including age, sex, type of intoxication ...

For induction of ruminal acidosis, 10 clinically healthy three years old non pregnant female sheep were selected. Prior to the infusion of sucrose (0 hour), rumen and blood samples were obtained in order to determine baseline rumen and blood pH, respectively. Electrocardiogram (ECG) was also recorded. Acute ruminal acidosis was induced experimentally with sucrose at a dose of 18g kg-1 body weig...

Metabolic acidosis secondary to lactic acidosis may occur in acute, severe asthma and its presence suggests that respiratory muscle fatigue and tissue hypoxia play a major part in the pathogenesis. Non-anion gap metabolic acidosis (NAG acidosis) has also been reported in acute asthma but its impact on the clinical outcome has not been evaluated. The objective of this study was to evaluate the p...

In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated the methylene chloride/methanol extract heartwood. They tested for in vitro protective effect against chromosome aberrations peripheral human lymphocytes, using cytokinesis-block micronucleus assay. All compounds (in minimal doses 1 μg/mL) exerted a beneficial by decreasing DNA damage lymphoc...

We examined in rats the effects of intraperitoneal angiotensin II (AII) infusion for 12 d on urinary excretion, plasma concentration, and in vitro release of prostaglandin (PG) E2 and 6-keto-PGF1 alpha, a PGI2 metabolite. AII at 200 ng/min increased systolic blood pressure (SBP) progressively from 125 +/- 3 to 170 +/- 9 mmHg (P less than 0.01) and elevated fluid intake and urine volume. Urinary...

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent tobacco-specific nitrosamine in animals and has been suggested to play a role in human tobacco-related cancers. Our previous study demonstrated that cytochrome P450 (P450) 1A2 catalyzes the formation of 4-hydroxy-1-(3-pyridyl)-1-butanone (keto alcohol) (an alpha-hydroxylation product) from NNK in human liver microsomes. Phenethyl ...