mda-9/Syntenin is a scaffolding PDZ domain-containing protein overexpressed in multiple human cancers that functions as a positive regulator of melanoma metastasis. Using a normal immortal human melanocyte cell line and weakly and highly metastatic human melanoma cell lines, we presently show that mda-9/syntenin initiates a signaling cascade that activates nuclear factor-KB (NF-KB) in human mel...

The malignant Reed-Sternberg cell of Hodgkin disease is an aberrant B cell that persists in an immunolgically mediated inflammatory infiltrate. Despite its nonproductive immunoglobulin genes, the ReedSternberg cell avoids the usual apoptotic fate of defective immune cells through an unknown mechanism. A likely candidate is the surface receptor, CD40, consistently expressed by Reed-Sternberg cel...

We have cloned two restriction enzyme fragments from the Hela cell line S3 which hybridize with the genomes of HPV-6 and 16 under conditions of reduced stringency (Tm-43°C). One of the fragments, a 5.7-kb Bam HI fragment, was found to be present within the other, a 15.8-kb Eco RI fragment. Restriction enzyme (RE) mapping of this 15.8-kb fragment (see Figure) revealed that the sequence of RE sit...

To avoid interpretative problems due to restriction fragment length polymorphisms, the monosomy 6 mutant cell line BM19.7 was employed to establish a molecular map of the human major histocompatibility (HLA) complex in the A2,B13,Bw4,DRw6,DRw52,DQw1,DPw2 haplotype. Results were obtained mainly by field-inversion gel electrophoresis and Southern blotting techniques. The map extends to 4800 kb an...

  Background :Adenosine is an endogenous purine nucleoside that has a neuromodulatory role in the central nervous system. The amphetamine derivative (±)-3,4-methylenedioxymethamphetamine (MDMA or ecstasy) is a synthetic amphetamine analogue used recreationally to obtain an enhanced affiliated emotional response. MDMA is a potent monoaminergic neurotoxin with the potential of damage to brain neu...

We have constructed a cDNA library from the mRNA of a rat liver cell line (BRL-3A) and characterized cDNA clones encoding the protein precursor of the rat insulin-like growth factor II (pre-pro-rIGF-II). This precursor, inferred from the nucleotide sequence, consists of a signal peptide, the rIGF-II sequence, and a trailer polypeptide of unknown significance. The characterized cDNA sequence (10...

Ann Surg Oncol. 2007 Dec;14(12):3620-8. Inhibition of proliferation by omega-3 fatty acids in chemoresistant pancreatic cancer cells. Hering J, Garrean S, Dekoj TR, Razzak A, Saied A, Trevino J, Babcock TA, Espat NJ. Department of Surgery, University of Illinois at Chicago, Chicago, Illinois, USA. BACKGROUND: Pancreatic cancer-gemcitabine (GEM) chemoresistance has been demonstrated to be associ...

Li, Yi-Ping, and Micheal B. Reid. NF-kB mediates the protein loss induced by TNF-a in differentiated skeletal muscle myotubes. Am J Physiol Regulatory Integrative Comp Physiol 279: R1165–R1170, 2000.—Nuclear factor-kB (NFkB) regulates the transcription of a variety of genes involved in immune responses, cell growth, and cell death. However, the role of NF-kB in muscle biology is poorly understo...

Background. Squamous cell carcinoma is a prevalent carcinoma of the oral cavity. Recently anti-proliferative effect of probiotics has been considered and assessed against different cancers. The aim of this study was to evaluate the cytotoxicity of Acetobacter syzygii strain supernatant on KB human oral cancer cell line and KDR human epithelial normal cell line. Methods. The cytotoxicity assessm...