The purpose of this study was to compare the embryo sexing methods between PCR and karyotyping of in vitro produced (IVP) bovine embryos at the 2-cell stage. The embryos were divided into 3 groups according to the time of first cleavage completion. Zygotes cleaving before 24 hours postinsemination (hpi), during 24-36 hpi and after 36 hpi were classified as fast, intermediate and slow cleaving e...

The cytogenetic findings were analysed in a series of 500 pregnancies in which chorionic villi sampling was performed. In all cases a direct method was used, karyotyping being successful in 481 cases (96.2%). The main indication for sampling was maternal age over 36 (412 cases; 82.4%). Abnormal laboratory findings resulted in 24 terminations of pregnancy (4.8%); in addition five unexpected bala...

The Philadelphia chromosome was the first genetic abnormality discovered in cancer (in 1960), and it was found to be consistently associated with CML. The description of the Philadelphia chromosome ushered in a new era in the field of cancer cytogenetics. Accumulating genetic data have been shown to be intimately associated with the diagnosis and prognosis of neoplasms; thus, karyotyping is now...

Partial trisomy 13q is an uncommon chromosomal abnormality with variable phenotypic expression. We report prenatal diagnosis of partial trisomy 13q in a fetus with partial agenesis of the cerebellar vermis, partial agenesis of the corpus callosum, hydrops and polyhydramnios. G-banding karyotyping, spectral karyotyping and array comparative genomic hybridization (aCGH) analysis of fetal blood we...

Natural occurrence of hexaploid loach Misgurnus anguillicaudatus detected in central China is reported here for the first time. The evidences from karyotyping, DNA content analysis and nuclear volume measurements were described to confirm the hexaploid nature of the identified individual.

Sensitivity tests based on a spectral nudging (SN) technique are conducted to analyze the effect of large-scale environmental factors on the movement of typhoon Megi (2010). The error of simulated typhoon track is effectively reduced using SN and the impact of dynamical factors is more significant than that of thermal factors. During the initial integration and deflection period of Megi (2010),...

We present a novel technique, intrinsic Raman spectroscopy (IRS), to correct turbidity-induced Raman spectral distortions, resulting in the intrinsic Raman spectrum that would be observed in the absence of scattering and absorption. We develop an expression relating the observed and intrinsic Raman spectra through diffuse reflectance using the photon migration depiction of light transport. Nume...

The purpose of this study was to examine the effect of loss of spectral detail on speech perception in Hebrew. Spectral smearing was carried out by multiplying the speech signal by a series of low-passed white noise samples, causing tonal components in the signal to be replaced by noise. Smearing bandwidths of 250Hz, 500Hz, 1000Hz, 2000Hz, 4000Hz, 8000Hz and full bandwidth were used. Smearing w...