Abstract The mangrove ecosystem in Tapak Village is one of the coastal areas Tugu District, Semarang, Central Java and close to industrial area. This presumed be contaminated by heavy metals. study aims analysis lead metal Tapak, isolate fungi from sediments, identify potential fungus according molecular approach. sampling method used a purposive random with 4 points. isolation was carried out ...

rhizoctonia zeae causes root rot and sheath blight on corn (zea mays) and several other gramineous plants. in order to determine the genetic diversity of the r. zeae population in mazandaran and golestan provinces, samples were collected from cultivatd soils of both provinces in 2011 and 2012.the identity of isolates as r. zeae were verified by amplification and sequencing of the 18s and 28s rd...

armillaria root and crown rot is an important disease of woody plants including fruit, forest and ornamental trees in different regions. the disease is common in isfahan and causes economical losses on a broad range of fruit trees. there only a few tolerant varieties to disease, and there is no efficient method in controlling the disease. therefore, detection of the pathogen from soil is necess...

ABSTRACT Visible and nonvisible quiescent infections of immature and mature fruit are an integral component of the disease cycle of brown rot of sweet cherry in California. Detection of these infections is critical for developing efficient and efficacious fungicide management programs. The previously published DNA amplification primers mfs3 and NS5 for the identification of Monilinia fructicola...

Increasing evidence of the fungal diversity in deep-sea sediments has come from amplification of environmental DNA with fungal specific or eukaryote primer sets. In order to assess the fungal diversity in deep-sea sediments of the Central Indian Basin (CIB) at ~5,000 m depth, we amplified sediment DNA with four different primer sets. These were fungal-specific primer pair ITS1F/ITS4 (internal t...

Late blight, a disease caused by oomycota, Phytophthora infestans, is greater threat to the potato crop than any other in Mauritius. This remains most challenging manage once symptoms have appeared, thus requiring rapid detection for effective management. The aim of this study was compare different methods early causal agent late blight. Conventional culture-based involved direct isolation P. i...

Introduction: Isolation of fungi from tissue specimens using conventional methods is time consuming. However, in some cases, the histopathological examination (HPE) alone unable to provide a definite identity fungus. Alternatively, non-culture method, such as polymerase chain reaction (PCR) detecting internal transcribed spacer (ITS) rRNA genes fungi, promising diagnostic tool for rapid and acc...

Ericaceae (the heath family) are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM) fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hym...

Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates and hosts other than onion, including chayote (S...