نتایج جستجو برای: its2 وrdna وpcr
تعداد نتایج: 1660 فیلتر نتایج به سال:
We sequenced the nuclear ribosomal internal transcribed spacer region 2 (ITS2) from several glassy-winged sharpshooter (GWSS) [Homalodisca vitripennis Germar (=H. coagulata Say)] egg parasitoid species (Hymenoptera: Mymaridae) belonging to the genus Gonatocerus Nees to test the utility of this fragment to confirm species boundaries and to define phylogenetic relationships. A total of 35 specime...
hyalomma and haemaphysalis are two most important genera of ticks (acari: ixodidae). the rapid and accurate identification of field collected specimens is crucial in faunal works, laboratory assays, anti-tick vaccine experiments, etc. the present study was designed to introduce a rapid and more sensitive method for the differentiation of closely related hyalomma and haemaphysalis species namely...
BACKGROUND Herbal drugs used to treat illness according to Ayurveda are often misidentified or adulterated with similar plant materials. OBJECTIVE To aid taxonomical identification, we used DNA barcoding to evaluate authentic and substitute samples of herb and phylogenetic relationship of four medicinal plants of family Asparagaceace and Asclepiadaceae. MATERIALS AND METHODS DNA extracted f...
A variety of genes have been used to type Pneumocystis carinii. In the present study, nucleotide sequence variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were used to type Pneumocystis carinii f. sp. hominis DNA obtained from the lungs of 60 human immunodeficiency virus-infected individuals. These regions were amplified by PCR, cloned, and sequenced. ...
Plants and their associated insect herbivores, represent more than 50% of all known species on earth. The first step in understanding the mechanisms generating and maintaining this important component of biodiversity is to identify plant-herbivore associations. In this study we determined insect-host plant associations for an entire guild of insect herbivores using plant DNA extracted from inse...
Anopheles pulcherrimus Theobald has a wide distribution in western Asia and is a potential vector of malaria in Iran. We have examined the rDNA-ITS2 (internal transcribed spacer 2) region of An. pulcherrimus specimens collected during the two peaks of activity (May-June and October-November) from Sistan and Baluchistan province, southeastern Iran. There were no consistent differences between sp...
anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...
زمینه وهدف: لیشمانیوز یک بیماری انگلی تک یاخته ای است که به عنوان یک معضل بهداشتی در بسیاری از کشورها مطرح است.هدف از این مطالعه، تشخیص عفونت انگل در انسان به منظور شناسایی اپیدمیولوژیک و ارائه برنامه کنترل با استفاده از پرایمر اختصاصی مشترک طراحی شده در سه گونه لیشمانیا است. مواد و روش ها:30 نمونه ایزوله شایع انگل لیشمانیا از مراکز مختلف در ایران جمع آوری شد. dna این ایزوله ها پس از کشت در محی...
زیره سیاه گیاه علفی و چندساله از خانواده چتریان است و رویشگاه طبیعی آن به ایران و تعداد کمی از کشورهای همسایه محدود میشود. با هدف ارزیابی تنوع ژنتیکی زیره سیاه ایران، نه اکوتیپ این گیاه از مناطق مختلف کشور جمعآوری شد و ناحیه its2 و همچنین ناحیه its1-5.8s rrna- its2 روی آنها تکثیر و تعیین توالی شد. توالیها به روش clustalw توسط نرمافزار megalign همردیف سازی شده و دندروگرام روابط فیلوژنیک و...
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