Isothermal titration calorimetry (ITC) has become increasingly popular because it is the only method that directly measures the enthalpy change for a reaction. When analysis is done properly and accurate thermodynamic values are obtained, ITC results can be compared to thermodynamic values collected by other methods and can be extrapolated to different conditions. However, incorrect assumptions...

The hexapeptide acetyl-Trp-Leu(5) (AcWL(5)) has the remarkable ability to assemble reversibly and spontaneously into beta-sheets on lipid membranes as a result of monomer partitioning followed by cooperative assembly. This system provides a unique opportunity to study the thermodynamics of protein folding in membranes, which we have done using isothermal titration calorimetry (ITC) and differen...

A comparative thermodynamic study of the interaction of anilinonaphthalene sulfonate (ANS) derivatives with bovine serum albumin (BSA) was performed by using differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC). The chemically related ligands, 1,8-ANS and 2,6-ANS, present a similar affinity for BSA with different binding energetics. The analysis of the binding driv...

Lysozyme-silica interactions and the resulting complexation were investigated through adsorption isotherms, dynamic and electrophoretic light scattering, circular dichroism (CD), and isothermal titration calorimetry (ITC). A thermodynamic analysis of ITC data revealed the existence of two binding modes during protein-nanoparticle complexation. Both binding modes are driven by the cooperation of...

Isothermal titration calorimetry (ITC) experiments were performed for investigation of binary mixtures comprised the Brønsted superbase DBN with hydrogen ethanoate (AcOH). The heat mixing (HE) profile was recorded at (343.15 ± 0.1) K and fitted a 5-parameter Redlich-Kister (RK) polynomial. RK fit parameters subsequently used to quantify partial molar heats mixing, xiHiE, each component i. ITC-b...

Melamine oligomers composed of repeating triazine-piperidine units and equipped with phenol phosphine oxide side-chains form H-bonded duplexes. The melamine backbone provides sufficient rigidity to prevent intramolecular folding up three recognition in length, leading reliable duplex formation between sequence complementary oligomers. NMR spectroscopy isothermal titration calorimetry (ITC) were...

Binding of hippuric acid (HA), a uremic toxin, with human serum albumin (HSA) has been examined by isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), molecular docking, circular dichroism (CD) and fluorescence spectroscopy to understand the reason that govern its impaired elimination through hemodialysis. ITC results shows that the HA binds with HSA at high (K b ~1...

ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding constants (K(b)) were measured by examining the systematic effect of ligand concentration on pro...

Forward osmosis (FO) is a promising technology for water treatment, but its fouling mechanisms are poorly understood compared to other membrane-based processes. This study focuses on combined caused by alginate (SA) and TiO2 nanoparticles, which serve as representative organic inorganic foulants, respectively. The results show that the co-presence of can effectively mitigate membrane SA under v...

Number of Papers published in peer-reviewed journals: Number of Papers published in non peer-reviewed journals: Final Report: High Stability Metal-Protein Interactions Evaluated by Microcalorimetry Report Title Isothermal titration calorimetry (ITC) is a microcalorimetry technique used to study interactions between biomolecules and ligands in aqueous solution. This ARO equipment grant was provi...