نتایج جستجو برای: isoenzyme of creatinine kinase
تعداد نتایج: 21192202 فیلتر نتایج به سال:
To the Editor: The last step in electrophoretic procedures for creatine kinase (CK; EC 2.7.3.2) isoenzyme separation on cellulose acetate is to incubate the plate with a reagent that will develop either fluorescence or color for each isoenzyme. In our laboratory we routinely use fluorescence to detect the isoenzymes. The reagent used must be highly sensitive, so that low proportions of the isoe...
In an effort to clarify the issue of potentially false increases in creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) in uremia, we evaluated the CK profile of 84 persons undergoing chronic maintenance hemodialysis. We compared the performance of a new commercial two-site chemiluminometric immunoassay of CK-MB (Magic Lite; Ciba Corning Diagnostics) with that of electrophoresis on agarose gel (C...
The prevalence of circulating macromolecular creatine kinase type 1 (macro CK type 1 or CK-immunoglobulin complexes) is significantly higher in a patient population selected for CK isoenzyme assay than in age- and sex-matched blood donors (n = 1304). In greater than 8000 patients studied, 49 individuals with macro CK type 1 were identified, yielding an overall prevalence of 0.61%. Macro CK type...
We report unusually high concentrations of free fatty acids and glycerol in sera of patients with adult-onset diabetes, and the accompanying alterations in creatine kinase isoenzyme MM patterns associated with such patients. The serum samples with increased free fatty acids also showed increased electrophoretic movement of the MM isoenzyme on cellulose acetate membranes. Fatty acid concentratio...
Laboratory data are critical to analyzing and improving clinical quality. In the setting of residual use of creatine kinase M and B isoenzyme testing for myocardial infarction, we assessed disease outcomes of discordant creatine kinase M and B isoenzyme +/troponin I (-) test pairs in order to address anticipated clinician concerns about potential loss of case-finding sensitivity following propo...
For about 30 years electrical cardioversion has been routine for converting arrhythmias. Whether the application of shocks in the usual dosage is safe is still controversial as minimal myocardial cell injury cannot be excluded. If simultaneous muscle damage occurs the measurement of common cardiac markers, especially creatine kinase and its isoenzyme creatine kinase MB, lacks specificity. We th...
2. Galen RS. The enzyme diagnosis of myocardial infarction. Hum Pathol1975;6:14155. 3. Lott JA. Creatine kinase, lactate dehydrogenase and their isoenzymes in clinical medicine. Corning, NY: Corning Medical, Corning Glass Works, 1984. 4. Yusuf S, Collins R, Lin L, Sterry H, Pearson M, Sleight P. Significance of elevated MB isoenzyme with normal creatine kinase in acute myocardial infarction. Am...
We report the effect of serum pH on the storage stability of the human creatine kinase isoenzymes and on the creatine kinase assay lag phase (Szasz et al., C/in. Chem. 22: 650, 1976). We also investigated the effect of including mercaptoethanol, N-acetyl-L-cysteine, monothioglycerol, ethylenediaminetetraacetate, or ethylene glycol bis(/3aminoethyl ether)-N,N,N#{231}N’-tetraacetate t 20, 4, and ...
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