Ion evaporation is an essential step in the formation of charged ions from electrosprays, yet many aspects of the process are poorly understood. The ion evaporation kinetics of the 1-ethyl-3-methyl-imidazolium+ (EMI+) based ionic liquids (ILs) EMI-BF4, EMI-bis(perfluoroethylsulfonyl)imide, EMI-bis(trifluoromethylsulfonyl)imide and EMI-tris(trifluoromethylsulfonyl)methide (EMI-Methide) are studi...

N-Linked glycans, extracted from patient sera and healthy control individuals, are analyzed by Matrix-assisted laser desorption ionization (MALDI) in combination with ion mobility spectrometry (IMS), mass spectrometry (MS) and pattern recognition methods. MALDI-IMS-MS data were collected in duplicate for 58 serum samples obtained from individuals diagnosed with Barrett's esophagus (BE, 14 patie...

RATIONALE Schindler disease is caused by the deficient activity of α-N-acetylgalactosaminidase, which leads to an abnormal accumulation of O-glycopeptides in tissues and body fluids. In this work the Schindler condition is for the first time approached by ion mobility (IMS) tandem mass spectrometry (MS/MS), for determining urine glycopeptide fingerprints and discriminate isomeric structures. ...

Glycopeptides from a tryptic digest of chicken ovomucoid were enriched using a simplified lectin affinity chromatography (LAC) platform, and characterized by high-resolution mass spectrometry (MS) as well as ion mobility spectrometry (IMS)-MS. The LAC platform effectively enriched the glycoproteome, from which a total of 117 glycopeptides containing 27 glycan forms were identified for this prot...

A hybrid atmospheric pressure ion mobility spectrometer is described which exhibits resolving power approaching the diffusion limit for singly and multiply charged ions (over 200 for the most favorable case). Using an electrospray ionization source and a downstream quadrupole mass spectrometer with electron multiplier as detector, this ESI-IMS-MS instrument demonstrates the potential of IMS for...

The progress of ion mobility spectrometry (IMS), together with its association to mass spectrometry (MS), opened new directions for the identification of various metabolites in complex biological matrices. However, glycolipidomics of the human brain by IMS MS represents an area untouched up to now, because of the difficulties encountered in brain sampling, analyte extraction, and IMS MS method ...

The use of ion mobility spectrometry systems to detect explosives in high security situations creates a need to determine compounds that interfere and may compromise accurate detection. This is the first study to identify possible interfering air contaminants common in airport settings by IMS. Seventeen suspected contaminants from four major sources were investigated. Due to the ionization sele...

Abstract The first High Kinetic Energy-Ion Mobility Spectrometry-Mass Spectrometry (HiKE-IMS-MS) studies involving six volatiles (acetone, acetonitrile, methanol, ethanol, 2-propanol, and 1-butanol) their fully deuterated analogues are reported. goal is to further our understanding of the ion–molecule chemistry occurring in HiKE-IMS. This needed for its full analytical potential be reached. Pro...