نتایج جستجو برای: inverse pcr
تعداد نتایج: 264411 فیلتر نتایج به سال:
Variable selection is an important tool in QSAR. In this article, we employ three known techniques: sliced inverse regression (SIR), principal components regression (PCR) and partial least squares regression (PLSR) for models to predict the boiling points of 530 saturated hydrocarbons. With 122 topological indices as input variables our results show that these three methods have good performanc...
cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5'- and 3'-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and proteins. Expressed Sequence Tag (EST) sequencing is the method of choice for recovering cDNAs represent...
B-cell functions as a key player in the humoral immunity producing immunoglobulin protein in mammalian. To produce wide-ranged and antigen-specific antibody, the B-cell undergoes genetic rearrangement of immunoglobulin genes during its maturation process. The genetic rearrangements in immunoglobulin genes require unstable steps for genome; breakage and re-ligation of the double strand DNA. In t...
Detecting the presence and diversity of low-level mutations in human tumors undergoing genomic instability is desirable due to their potential prognostic value and their putative influence on the ability of tumors to resist drug treatment and/or metastasize. However, direct measurement of these genetic alterations in surgical samples has been elusive, because technical hurdles make mutation dis...
A new approach for simultaneously amplifying the 5' and 3' ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5' and 3' regions of a certain cDNA with very low or no background. It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flankin...
Large insert mate pair reads have a major impact on the overall success of de novo assembly and the discovery of inherited and acquired structural variants. The positional information of mate pair reads generally improves genome assembly by resolving repeat elements and/or ordering contigs. Currently available methods for building such libraries have one or more of limitations, such as relative...
The momenta of τ decay products in the reaction e + e − → τ + τ − do not constrain the τ direction unambiguously. It is shown how the measurement of tracks of hadrons from semileptonic τ decays, in particular their relative impact parameters, allows to resolve this ambiguity.
Robotica / Volume 34 / Issue 08 / August 2016, pp 1734 1753 DOI: 10.1017/S0263574716000138, Published online: 13 April 2016 Link to this article: http://journals.cambridge.org/abstract_S0263574716000138 How to cite this article: Jin Seob Kim and Gregory S. Chirikjian (2016). Inverse kinematic solutions of 6-D.O.F. biopolymer segments. Robotica, 34, pp 1734-1753 doi:10.1017/S0263574716000138 Req...
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