In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injec...

BACKGROUND Generally, intracytoplasmic sperm injection (ICSI) may be the preferable method to treat partial globozoospermia, but whether there exist some correlations between ICSI fertilization rate and the proportion of round-headed sperm or morphologically normal sperm remains open. This study was to explore the correlation between ICSI fertilization rate and the sperm morphology in patients ...

In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At 10 h after ICSI or IVF, we selected only zygo...

I n the recent literature, several interesting articles have been published using the zona pellucdia (ZP)-bound sperm for intracytoplasmic sperm injection (ICSI) to enhance embryo quality, implantation and clinical pregnancy rates. The three studies were performed independently in different ART clinics. In the test group, patients’ sibling immature (either germinal vesicle (GV) or metaphase I) ...

OBJECTIVE The aim of this study is assessment of effects of different assisted reproductive techniques (ART) like in vitro fertilization (IVF) and intra cytoplasmic sperm injection (ICSI) on prelinguistic behavior of infants conceived by these techniques. METHODS In this descriptive, cross sectional study, prelinguistic behavior of 151 full term ART infants of Royan Institute have been assess...

Background: There is increasing evidence that psychological factors like anxiety and depression can affect IVF/ICSI treatment results. Objective: This study aimed to clarify the role of women’s anxiety and depression on the outcome of ART cycles using Intra Cytoplasmic Sperm Injection (ICSI). Materials and Methods: This was a prospective pilot study. One hundred six (106) consecutive women unde...

BACKGROUND Intracytoplasmic Sperm Injection (ICSI) is an Assisted Reproduction Technique (ART) which offers the chance to conceive to patients presenting very low sperm counts (cryptozoospermia/severe oligozoospermia). Sperm freezing before the oocyte pick-up, can prevent from a lack of spermatozoa on the day of the ICSI. It can avoid the cancellation of the ICSI or the use of TESE (Testicular ...

Background Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some...

the main role of cumulus cells (cc) is to provide nutritious materials for developing oocytes. follicular fluid (ff) contains the enzyme acid phosphatase, which plays a role in ovulation and fertilization of oocyte. the objective of this prospective study was to evaluate the levels of acid phosphatase in ff and the rate of apoptosis in cc of fertilized and unfertilized oocytes in intracytoplasm...

Background: During spermatogenesis the sperm nucleus exchange histones against cystein rich protamines, which stabilize the sperm chromatin and protects the DNA against damages. At ejaculation the age of the patient, handling routines, processing of the semen sample and the storage of the final sperm suspension might damage the DNA. Sperm chromatin stability and DNA damages are evaluated with d...