Using a multiprobe RNase protection assay, we examined cytokine and chemokine mRNAs that were expressed after corneal infection with Pseudomonas aeruginosa in mice. Cytokines that were upregulated included interleukin-1alpha (IL-1alpha) and -1beta, IL-1 receptor antagonist, IL-6, IL-11, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, macrophage colony-st...

Introduction: Epstein-Barr virus positive (EBV+) lymphoproliferative disorders (LPD) with Hodgkin-like features include indolent, localised conditions such as EBV+ mucocutaneous ulcer (EBVMCU) through to systemic lymphomas, classical Hodgkin lymphoma (EBVcHL) and diffuse large B-cell (EBVDLBCL). The diagnostic distinction between these LPD is imperative guide management, but can prove problemat...

Cytokine-activated neutrophils are known to be essential for protection against group A Streptococcus infections. However, during severe invasive group A Streptococcus infections that are accompanied by neutropenia, it remains unclear which factors are protective against such infections, and which cell population is the source of them. Here we show that mice infected with severe invasive group ...

Aim: To compare the frequency of thyroid disorders in patients of chronic hepatitis C being treated with interferon alpha versus pegylated interferon. Methods:: This randomized Controlled Trial was conducted at Department of Medicine, Bahawal Victoria Hospital, Bahawalpur from October 2014 to April 2015.A total of 340 diagnosed case of chronic hepatitis C by PSR HCV RNA of both genders, having ...

We have previously shown that the human myeloid cell nuclear differentiation antigen (MNDA) is expressed at both the antigen and mRNA levels specifically in human monocytes and granulocytes and earlier stage cells in the myeloid lineage. A 200 amino acid region of the MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-2...

a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...

Both genetic and environmental interactions affect systemic lupus erythematosus (SLE) development and pathogenesis. One known genetic factor associated with lupus is a haplotype of the interferon regulatory factor 5 (IRF5) gene. Analysis of global gene expression microarray data using gene set enrichment analysis identified multiple interferon- and inflammation-related gene sets significantly o...

The 3′ repair exonuclease 1 (TREX1) gene encodes a nuclear protein with activity, and the mutations have been associated autoimmune diseases. Herein, we performed genetic analysis for TREX1 in 55 patients systemic lupus erythematosus (SLE). We identified one SLE patient overlapping dermatomyositis having heterozygous p.Asp130Asn mutation gene. had high level of serum interferon (IFN)-α compared...

Systemic lupus erythematosus is a diffuse connective tissue disease mediated by autoimmunity and characterized immune inflammation. The purpose of this study to explore candidate key genes cell infiltration involved in the pathogenesis systemic erythematosus. Intersection derived from differentially expressed between patients healthy individuals are identified weighted gene co-expression networ...