نتایج جستجو برای: integrase enzyme
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Integration of the HIV-1 cDNA into the human genome is catalyzed by the viral integrase (IN) protein. Several studies have shown the importance of cellular cofactors that interact with integrase and affect viral integration and infectivity. In this study, we produced a stable complex between HIV-1 integrase, viral U5 DNA, the cellular cofactor LEDGF/p75 and the integrase binding domain of INI1 ...
Background HIV integrase (IN) plays important roles at several steps, including viral DNA nuclear import, targeting viral DNA to host chromatin and integration. Identification of novel inhibitors of HIV Integrase has emerged as promising antiviral agents for the treatment of HIV/AIDS. Present work is to investigation of anti-HIV activity and HIV integrase inhibitory activity of various extracts...
background: the aim of this study was to produce a stable cho cell line expressing tenecteplase. materials and methods: in the first step, the tenecteplase coding sequence was cloned in a pdb2 vector containing attb recognition sites for the phage φc31 integrase. then, using lipofection, the cho cells were co-transfected with constructed recombinant plasmid encoding tenecteplase and attb recogn...
Azadirachta indica (Neem) is an evergreen tree that belongs to the Meliaceae family. It native Indian subcontinent and grows worldwide. also known as "village pharmacy" in India for its wide range of therapeutic pharmacological properties. An vitro study indicated A. showed anti-HIV However, exact mechanism supposed properties remains unknown. This aimed construct in-silico database compounds p...
The degree of sequence variation in HIV-1 integrase genes among infected patients and their impact on clinical response to Anti retroviral therapy (ART) is of interest. Therefore, we collected plasma samples from 161 HIV-1 infected individuals for subsequent integrase gene amplification (1087 bp). Thus, 102 complete integrase gene sequences identified as HIV-1 subtype-C was assembled. This sequ...
We report the activities of HIV integrase protein on a novel DNA substrate, consisting of a pair of gapped duplex molecules. Integrase catalyzed an intermolecular disintegration reaction that requires positioning of a pair of the gapped duplexes in a configuration that resembles the intgration intermediate. However, the major reaction resulted from an intramolecular reaction involving a single ...
Computational screening of inhibitors for HIV-1 integrase using a receptor based pharmacophore model
The HIV (human immuno-deficiency virus) integrase has a crucial role in viral replication. Moreover, it has no cellular homologue in humans. Hence, it is considered as an attractive drug target. Many inhibitors against the integrase protein has been designed and discussed. The Y-3 inhibitor (4-acetyl amino-5-hydroxy naphthalene - 2, 7- disulfonic acid) is already known to inhibit HIV-1 integras...
We found in the environmental strain Nitrosomonas europaea a chromosomal integron-like structure with an integrase gene, intI(Neu). We have tested the capacity of the IntINeu integrase to excise and integrate several resistance gene cassettes. The results allow us to consider IntINeu a new functional integron integrase.
Phage integrase genes often play a role in the establishment of lysogeny in temperate phage by catalyzing the integration of the phage into one of the host's replicons. To investigate temperate phage gene expression, an induced viral metagenome from Tampa Bay was sequenced by 454/Pyrosequencing. The sequencing yielded 294,068 reads with 6.6% identifiable. One hundred-three sequences had signifi...
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