A highly sensitive spectroscopic method using dichlorofluorescin (LDCF) was employed to study the rate of electron-transfer reaction in presence of dsDNA, ssDNA and some metal ions and imidazole derivative (N-trans cinnamoyl imidazole).Our results show that both kinds of DNA possess an enzyme-like catalytic activity in oxidative conversion of non fluorescent LDCF to fluorescent DCF. A biphasic ...

The phototoxicity mechanism of a kryptocyanine dye, N,N'-bis(2-ethyl-1,3-dioxolane)kryptocyanine (EDKC+), has been studied in RBC membranes and isolated mitochondria. Lipophilic, positively charged dyes, such as EDKC+, may be useful as tumor-cell-selective, light-activated cytotoxic agents. Exposure of the RBC membranes to 700-nm light and EDKC+ inhibited membrane acetylcholinesterase and photo...

The initiation of electron transfer by addition of substrate to nonphosphorylating preparations has been recorded by a number of workers and is generally accepted to be a rapid reaction. Polarographic measurements of oxygen utilization on addition of succinate to Keilin and Hartree heart muscle preparations and spectrophotometric recordings of fumarate formation in the same system support this ...

the major objectives of this study were to find out the mechanism behind the sapstaining fungal hyphae penetration into wood elements using electron microscope and to evaluate the exact role of phenolic compounds existing in wood vinegar towards the sapstaining fungal growth inhibition. to formulate the new environmental friendly wood preservative, we neutralized (to ph 7) wood vinegar with nao...

In this review, we discuss the factors that influence electron transfer in peptides. We summarize experimental results from solution and surface studies and highlight the ongoing debate on the mechanistic aspects of this fundamental reaction. Here, we provide a balanced approach that remains unbiased and does not favor one mechanistic view over another. Support for a putative hopping mechanism ...

Electron-transfer (ET) reactions are key steps in a diverse array of biological transformations ranging from photosynthesis to aerobic respiration. A powerful theoretical formalism has been developed that describes ET rates in terms of two parameters: the nuclear reorganization energy (lambda) and the electronic-coupling strength (HAB). Studies of ET reactions in ruthenium-modified proteins hav...

In most of the oxidation—reduction reactions involving net electron transfer which have been studied, the experimental results yield only second order rates, and thus reflect the barrier to bringing reactants together as well as that for the electron transfer act itself. Recent advances in synthetic procedures make it possible to measure as an intramolecular or first order process, net electron...