Infectious bronchitis (IB) is a very contagious disease caused by a coronavirus (IBV). In chickens, thevirus affects the respiratory, reproductive, and urinary systems. This study was carried out to determine theseroprevalence of anti-IBV antibodies in domestic village chickens. Serum samples of 300 domestic villagechickens from Esfahan (centeral Iran) were collected and examined for the presen...

S OF PAPERS 239 101 Genome sequencing and comparative analysis of avian coronavirus. M. W. Jackwood,* E. T. McKinley, J. E. Philips, and S. W. Thor, University of Georgia, Athens. Genome sequencing and comparative genomics can be used to examine past host and tissue tropism shifts to aid in identification of new variant viruses, to identify genetic differences between different pathotypes, and ...

OBJECTIVE Garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. Infectious bronchitis virus (IBV) is a coronavirus. The available vaccines against IBV cannot cover new variants. This study evaluated the inhibitory effects of garlic extract on IBV. MATERIALS AND METHODS The constituents of garlic extract were detected by gas chromatography. This study was do...

We have previously demonstrated that the replacement of the S gene from an avirulent strain (Beaudette) of infectious bronchitis virus (IBV) with an S gene from a virulent strain (M41) resulted in a recombinant virus (BeauR-M41(S)) with the in vitro cell tropism of the virulent virus but that was still avirulent. In order to investigate whether any of the other structural or accessory genes pla...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...

It is important to rapidly differentiate infectious bronchitis virus (IBV) from disease agents like highly pathogenic avian influenza virus and exotic Newcastle disease virus, which can be extremely similar in the early stages of their pathogenesis. In this study, we report the development and testing of a real-time RT-PCR assay using a Taqman-labeled probe for early and rapid detection of IBV....

  In order to identify predominant circulating types of infectious bronchitis(IB) virus in the period of 1999-2004 in Iran, samples(trachea, lung, kidney) from 150 various flocks showing respiratory signs suspected to be related to IB infection were inoculated to SPF eggs. Then allantoic fluids studied by RT-PCR and Nested PCR using group and type specific primers to probing Massachusset, 793/B...

Four-day-old specific-pathogen-free chickens were inoculated by eyedrop with four different strains (Gray, JMK, CV56b, and Wolgemuth) of infectious bronchitis virus (IBV). Birds were monitored clinically and euthanatized at 1, 4, 7, and 14 days postinfection and tissues were collected for virus isolation, histopathologic examination, in situ hybridization (ISH), and immunohistochemistry (IHC). ...

BACKGROUND Melanoma differentiation associated gene 5 (MDA5) and retinoic acid-inducible gene-I (RIG-I) selectively sense cytoplasmic viral RNA to induce an antiviral immune response. Infectious bronchitis virus (IBV) is one of the most important infectious agents in chickens, and in chicken cells, it can be recognized by MDA5 to activate interferon production. RIG-I is considered to be absent ...

Avian infectious bronchitis virus (IBV) causes high mortality and reduced production even in vaccinated birds. The study hypothesis was that a decoction of a Chinese herbal medicine, modified Ma Xing Shi Gan Tang, in the drinking water would reduce mortality and improve recovery and antioxidant capabilities in chickens infected with IBV. Two hundred 15-day-old chickens were randomly divided int...