Introduction: Alpha-Momorcharin (α-MMC) is a member of the ribosome-inactivating protein (RIP) family that has been widely used as an antitumor, antiviral and antifungal agent. Methods: In this study, codons DNA encoding α-MMC were optimized for expression in E. coli cloned into pET-28a(+) vector. The was then expressed strain BL21 (DE3) purified by nickel affinity chromatography. Results: Unde...