OBJECTIVE To produce the monoclonal antibodies (mAbs) against hygromycin B phosphotransferase (HPT) and to develop immunoassay based on mAbs for biosafety assessment of HPT in genetically modified rice (GM rice). METHODS BALB/c mice were immunized with purified recombinant 6His. HPT protein, and the conventional hybridoma technology was used to generate the monoclonal hybridoma cells. ELISA a...

Rift Valley fever (RVF) virus is an important zoonotic and a potential biothreat agent. This paper describes validation of sandwich and capture enzyme-linked immunoassays (ELISA) based on gamma-irradiated antigens for the detection of RVFV-specific IgG and IgM antibody in humans. Validation data sets derived from testing field-collected sera from Africa (n=2400) were dichotomised according to t...

BACKGROUND In late 2011, a new Orthobunyavirus of the Simbu serogroup named Schmallenberg virus (SBV) emerged in continental Europe. The virus is transmitted by hematophagous arthropods, with the Culicoides species as, so far known, main vectors. Infection with the virus can cause clinical signs in adult ruminants including diarrhea, fever and reduced milk production. Transplacental infection o...

BACKGROUND Malaria control efforts are limited in rural areas. A low-cost system to monitor response without the use of electricity is needed. Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. A three-part system using ELISA was developed consisting of a microfluidic chip, hand crank centrifuge, and a smartphone. METHODS A circular...

Kallikrein-related peptidases (KLKs) have been proposed as potential cancer biomarkers. Kallikreinrelated peptidase 5 (KLK5) is a secreted trypsin-like protease of the KLKs. Until now, detection of KLK5 in both biological fluids and tissues has been described frequently due to the potential of being a new cancer biomarker. Our objective was to prepare KLK5 antibodies and establish an ELISA meth...

conclusions the present findings reveal a relatively high presence of anti-hev iggs and clearly indicate that hev infection is still present in northern algeria. further, the prediction of hev genotype using different antigens generated from the different hev genotypes shows that the causative strains are more likely to be of genotype 1. background two hepatitis e virus (hev) outbreaks occurred...

hydatidosis, caused by the larval stage of echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. as its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. sera (250 samples) were collected from 90 cases of hydatidosis pro...

BACKGROUND Plasma phospholipid transfer protein (PLTP) plays a central role in the remodeling of HDLs. Reliable and accurate methods for assaying PLTP concentration are required. METHODS A sandwich ELISA for PLTP has been developed, using two monoclonal antibodies against recombinant human PLTP (rhPLTP) expressed in Chinese hamster ovary cells. The ELISA allows for the quantification of PLTP ...