Bone marrow aspirates from 306 patients with multiple myeloma were analyzed by flow cytometric immunophenotyping. The plasma cells (PCs) were identified by their characteristic light scatter distribution and reactivity patterns to CD138, CD38, and CD45. Monoclonality was confirmed by immunoglobulin light chain analysis. The immunophenotypic profile of the PCs was determined with a panel of anti...

Hematopathology is a rapidly evolving subspecialty in which diagnosis often involves integration of morphology, immunophenotyping, cytogenetics, and molecular data. Many of the recent changes are highlighted in the 2016 revision to the 4th edition of the WHO classification of tumours of hematopoietic and lymphoid tissue. Lineage (i.e. B-cell, T-cell, NK-cell) and maturation (immature vs. mature...

Azurophilic granulation of blasts is a feature of acute myeloid leukemia (AML). Granular acute lymphoblastic leukemia (ALL) may mimic AML due to the presence of cytoplasmic granules in lymphoblasts, but cytochemistry and immunophenotyping are helpful in making the correct diagnosis. Toluidine blue (TB) is a metachromatic dye, which stains basophils and myeloid blasts that exhibit basophilic dif...