BACKGROUND The uterus is a dynamic tissue responding to hormonal changes during reproductive cycles. As such, uterine stem cells have been studied in recent years. Transcription factors oct4 and sox2 are critical for effective maintenance of pluripotent cell identity. OBJECTIVE The present research evaluated the mRNA expression of oct4 and sox2 in the uterine tissues of ovariectomized mice tr...

The peripheral blood and bone marrow samples from 50 patients with ALL were investigated by indirect immunofluorescent technique. The most common type of ALL was common-ALL (82%) and the least common was T-ALL (2%). Other subtypes of ALL were unclassified by our technique (8%), B-ALL (4%), and pre B-ALL (4%). The correlation between immunophenotype, clinical condition and hematological pro...

SYNOPSIS Immunofluorescent serum IgM and/or a significant level of IgA antibody was detected in 87 % of 39 cases of current or recent influenza A infection from two to 84 days after the onset of illness. Secondary IgM staining occurred in 5% of sera and a significant correlation was found between complement-fixing and class-specific antibodies. It was estimated that the immunofluorescent test c...

This study estimated the sensitivity and specificity of the rK39 strip test compared with the immunofluorescent antibody test and microscopy of bone marrow aspirate smears (the gold standard) in 47 children with suspected visceral leishmaniasis. A control group of children with other diagnoses (tuberculosis, toxoplasmosis, systemic lupus erythematosus, malaria or cutaneous leishmaniasis) were a...

Immunofluorescent serum IgM and/or a significant level of IgA antibody was detected in 87% of 39 cases of current or recent influenza A infection from two to 84 days after the onset of illness. Secondary IgM staining occurred in 5% of sera and a significant correlation was found between complement-fixing and class-specific antibodies. It was estimated that the immunofluorescent test could be di...

Serum factors reactive with human heart or extracts of human heart have been found by a number of investigators utilizing a variety of immunologic techniques (1-7). Kaplan, Meyeserian, and Kushner (8) have demonstrated reactive factors in the sera of patients with acute rheumatic fever and certain other diseases by an immunofluorescent technique. The present study, using the immunofluorescent m...

alpha2-Macroglobulin (alpha2M) has been identified on the luminal surface of endothelial cells in sections of normal human arteries, veins, and lymphatics by the indirect immunofluorescent technique. The specificity of the immunofluorescent reaction was confirmed by immunoabsorption studies. Prior absorption of the anti-alpha2M antiserum by purified alpha2M at equivalence completely inhibited e...

Electron microscopic and immunofluorescent studies are reported in a patient with idiopathic pulmonary hemosiderosis, suggesting that idiopathic pulmonary hemosiderosis is distinct from other pulmonary hemorrhagic syndromes associated with antibasement membrane antibody or immune complex mediated diseases. The ultrastructural studies showed evidence of nonspecific lung injury and indirect evide...

Cover Illustration: The 3D reconstruction of super-resolution immunofluorescent confocal image shows that glutamate transporter 1 (GLT1; green)-labeled astrocytic processes enwrap neurons labeled by microtubule-associated protein 2 (MAP2, red; nuclei, blue) in primary mouse cortical glia-neuron mix culture. close proximity MAP2-GLT1 indicates the neuron-astrocyte interaction for maintaining hom...

Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-...