نتایج جستجو برای: immunoassays

تعداد نتایج: 5308  

Journal: :Clinical chemistry 2005
Susann Eriksson Heidi Halenius Kari Pulkki Jukka Hellman Kim Pettersson

BACKGROUND There are numerous potential sources of interference in immunoassays. Our aim was to identify the blood component that causes negative interference in cardiac troponin I (cTnI) immunoassays based on antibodies against the central part of cTnI. METHODS We isolated an interfering factor (IF) from a sample with low recovery of added cTnI, using several consecutive purification steps: ...

Houshang Amirrasouli, Faranak Kazerouni,

Background: Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resu...

2017
Rachel A. Bartholomew Richard M. Ozanich Jennifer S. Arce Heather E. Engelmann Alejandro Heredia-Langner Beth A. Hofstad Janine R. Hutchison Kristin Jarman Angela M. Melville Kristin D. Victry Cynthia J. Bruckner-Lea

There is little published data on the performance of biological indicator tests and immunoassays that could be used by first responders to determine if a suspicious powder contains a potential biothreat agent. We evaluated a range of biological indicator tests, including 3 protein tests, 2 ATP tests, 1 DNA test, and 1 FTIR spectroscopy instrument for their ability to screen suspicious powders f...

Journal: :Analytica chimica acta 2017
Hyo-Deok Seo Joong-Jae Lee Yu Jung Kim Oliver Hantschel Seung-Goo Lee Hak-Sung Kim

Enzyme-linked immunoassays based on an antibody-antigen interaction are widely used in biological and medical sciences. However, the conjugation of an enzyme to antibodies needs an additional chemical process, usually resulting in randomly cross-linked molecules and a loss of the binding affinity and enzyme activity. Herein, we present the development of an alkaline phosphatase-fused repebody a...

2003
Anthony R. Mire-Sluis

Five ampouled preparations of interleukin-4 (IL-41 have been evaluated by 36 laboratories in 14 countries for their suitability to serve as an international standard for this material in a joint international collaborative study for interleukin-3 (IL-3) and IL-4. The preparations were assayed in a wide range of in vitro bioassays and immunoassays. It is clear from the study that different recom...

Journal: :Seminars in thrombosis and hemostasis 2012
Chee Wee Tan Christopher Morice Ward Marie-Christine Morel-Kopp

Heparin-induced thrombocytopenia (HIT) is a rare but potentially serious complication of heparin use. Prompt diagnosis is crucial and requires the integration of clinical assessment and laboratory testing. Pretest clinical scoring systems (i.e., 4 Ts) have been established. Immunoassays can detect the presence of antibodies directed toward heparin-platelet factor 4 (H-PF4) complexes, but provid...

Journal: :Analytical chemistry 2013
Kihwan Choi Alphonsus H C Ng Ryan Fobel David A Chang-Yen Lyle E Yarnell Elroy L Pearson Carl M Oleksak Andrew T Fischer Robert P Luoma John M Robinson Julie Audet Aaron R Wheeler

We introduce an automated digital microfluidic (DMF) platform capable of performing immunoassays from sample to analysis with minimal manual intervention. This platform features (a) a 90 Pogo pin interface for digital microfluidic control, (b) an integrated (and motorized) photomultiplier tube for chemiluminescent detection, and (c) a magnetic lens assembly which focuses magnetic fields into a ...

2012
Bhupal Ban Diane A. Blake

The measurement of trace amounts of physiologically active small molecules (for example, lipids, drugs, other synthetic chemicals and metals) is critical for both clinical and environmental analyses. Most small molecules can be analyzed using highly sophisticated analytical techniques, including high pressure liquid chromatography (HPLC), gas chromatography (GC), and inductively coupled plasma ...

2018
Xuzhen Qin Jianzhong Rui Yong Xia Hong Mu Sang Hoon Song Raja Elina Raja Aziddin Gabrielle Miles Yuli Sun Sail Chun

BACKGROUND The immunosuppressant drugs (ISDs), tacrolimus and cyclosporine, are vital for solid organ transplant patients to prevent rejection. However, toxicity is a concern, and absorption is highly variable across patients; therefore, ISD levels need to be precisely monitored. In the Asia-Pacific (APAC) region, tacrolimus and cyclosporine concentrations are typically measured using immunoass...

Journal: :The Analyst 2014
A Szkola E M Linares S Worbs B G Dorner R Dietrich E Märtlbauer R Niessner M Seidel

Simultaneous detection of small and large molecules on microarray immunoassays is a challenge that limits some applications in multiplex analysis. This is the case for biosecurity, where fast, cheap and reliable simultaneous detection of proteotoxins and small toxins is needed. Two highly relevant proteotoxins, ricin (60 kDa) and bacterial toxin staphylococcal enterotoxin B (SEB, 30 kDa) and th...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید