Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is sweeping the world since end of 2019. The titer change antibodies against SARS-CoV-2 needs to be further clarified, clinical and preventive value still investigated. An enzyme-linked immunosorbent assay (ELISA) was established by coating with recombinant spike protein used detect serum immunoglobulin M (IgM) G (IgG) in disease 2019...

Detection of IgG and IgM antibodies is commonly performed for the diagnosis of infection with Toxoplasma gondii. We determined the accuracy of the Elecsys Toxo IgG and IgM test at four European laboratories compared to local reference methods. Coefficients of variation for reproducibility ranged from 1.0 to 6.5% for IgG and from 0.8 to 3.2% for IgM. Seroconversion panels revealed high overall c...

Although glomerular immunoglobulin M (IgM) deposition occurs in a variety of glomerular diseases, the mechanism of deposition and its clinical significance remain controversial. Some have theorized IgM becomes passively trapped in areas of glomerulosclerosis. However, recent studies found that IgM specifically binds damaged glomeruli. Therefore, we tested whether natural IgM binds to neo-epitop...

The seroprevalence of toxoplasmosis in 832 pregnant women in Miracema, Rio de Janeiro, was determined and 75.1% (625) and 2.0% (17) were anti-Toxoplasma gondii IgG and IgM positive, respectively. Out of the 17 IgM positive pregnant women, only one had low avidity IgG corresponding to the acute phase of the infection. All the other women presented with high avidity IgG and also presented with re...

An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo ...

Background: IgG antibodies specific for the SARS-CoV-2 Spike protein are under intensive investigation urgent need to identify a correlate of protective immunity in individuals vaccinated with licensed and candidate COVID-19 vaccines. Limited data available on vaccine-elicited IgM their potential implication SARS-CoV-2. Methods: We performed longitudinal study quantify spike (IgG-S IgM-S) 1873 ...