The Abbott RealTime HIV-1 assay is an automated test for monitoring HIV-1 viral load in plasma samples. The assay uses reverse transcription polymerase chain reaction (RT-PCR) technology with homogeneous real-time fluorescent detection. Automated sample preparation is performed on the m2000sp instrument where RNA is isolated using magnetic microparticle technology and dispensed to a PCR tray to...

background: the number of interventional cardiology (ic) procedures has increased rapidly. coronary angiography (ca) and percutaneous transluminal coronary angioplasty (ptca) are now widely performed as a matter of routine, and they are considered safe procedures for experienced cardiologists. however, it is also known that these procedures are associated with high radiation doses due to long f...

A single-step, multiplex, real-time polymerase chain reaction (RT-PCR) was developed for the simultaneous and differential laboratory diagnosis of Classical swine fever virus (CSFV) and African swine fever virus (ASFV) alongside an exogenous internal control RNA (IC-RNA). Combining a single extraction methodology and primer and probe sets for detection of the three target nucleic acids CSFV, AS...

Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per ...

PURPOSE The role of IL28B gene variants and expression in hepatitis B virus (HBV) infections are not well understood. Here, we evaluated whether IL28B gene expression and rs12979860 variations are associated with HBV outcomes. MATERIALS AND METHODS IL28B genetic variations (rs12979860) were genotyped by pyrosequencing of DNA samples from 137 individuals with chronic HBV infection [50 inactive...

Dengue has become a global public health problem and a sensitive diagnostic test for early phase detection can be life saving. An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R(2) = 0.9967) and a LOD of at least 1.95 × 10(4) copies...

In IMpassion050 (NCT03726879), adding atezolizumab (A) to neoadjuvant chemotherapy (CT) and pertuzumab–trastuzumab (PH) did not increase pathological complete response (pCR) vs placebo (Pl), CT PH in patients (pts) with high-risk, HER2-positive (+) early breast cancer (EBC) either the intention-to-treat or PD-L1+ populations. Additional BM data are warranted better inform future immunotherapeut...

Given a coloring f : V (G) → IN of graph G and any subgraph H ⊂ G we define fs(H) = ∑ v∈V (H) f(v). In particular, we denote fs(G) by S(f). The coloring f is called an ICcoloring if for any integer k ∈ [1, S(f)] there is a connected subgraph H ⊂ G such that fs(H) = k. Also, we define the IC-index of G to be M(G) = max{ S(f) : f is an IC-coloring of G }. In this paper we examine some well-known ...

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

Abstract The bacterium Avibacterium paragallinarum , previously known as Haemophilus is responsible for causing infectious coryza (IC) in chickens and other avian species. In this case report, an outbreak of occurred the Qinling area China, resulting clinical symptoms facial swelling several bird species, including Golden pheasant, Temminck's tragopan, Peafowls, three pheasants died due to prol...