Dendritic cells (DC) are professional antigen-presenting cells that are used in vaccine approaches to cancer. Classically, mature monocyte-derived DC are generated in vitro in the presence of interleukin (IL)-4, granulocyte macrophage-colony stimulating factor, and inflammatory cytokines (G4-DC). Recently, it has been described that DC can also be generated in the presence of IL-3 and interfero...

Nine healthy volunteers were studied on the seventh day of dosing at 21:00 h with nizatidine 150 mg (N 150), nizatidine 300 mg (N 300), ranitidine 300 mg (R 300), or placebo, given in a predetermined random order. The double-blind 24 hour studies, using the Royal Free Hospital standard protocol, simultaneously measured intragastric acidity and plasma gastrin concentration. Compared with placebo...

MOTIVATION Eukaryote-infecting nucleo-cytoplasmic large DNA viruses (NCLDVs) feature some of the largest genomes in the viral world. These viruses typically do not strongly depend on the host DNA replication systems. In line with this observation, a number of essential DNA replication proteins, such as DNA polymerases, primases, helicases and ligases, have been identified in the NCLDVs. One oth...

The purpose of this presentation is to outline the Museum Multimedia (MM) experience in Russia. I am quite not sure wether all my colleagues experts and museum community in West Europe and America are intimately familiar with Russian experience in this sphere and I am pleased to have this opportunity to fill the gap. I will mainly focus on the general picture for some more reports with detailed...

Various heterotrimeric G(i) proteins are considered to be involved in cell migration and effector function of immune cells. The underlying mechanisms, how they control the activation of myeloid effector cells, are not well understood. To elucidate isoform-redundant and -specific roles for Gα(i) proteins in these processes, we analyzed mice genetically deficient in Gα(i2) or Gα(i3). First, we sh...

The molecular composition and organization of the row of axonemal inner dynein arms were investigated by biochemical and electron microscopic analyses of Chlamydomonas wild-type and mutant axonemes. Three inner arm structures could be distinguished on the basis of their molecular composition and position in the axoneme as determined by analysis of pf30 and pf23 mutants. The three inner arm stru...

We prove the following theorems: 1) The Laurent expansions in ε of the Gauss hypergeometric functions 2F1(I1 + aε, I2 + bε; I3 + p q + cε; z), 2F1(I1 + p q + aε, I2 + p q + bε; I3+ p q + cε; z) and 2F1(I1+ p q +aε, I2+ bε; I3 + p q + cε; z), where I1, I2, I3, p, q are arbitrary integers, a, b, c are arbitrary numbers and ε is an infinitesimal parameter, are expressible in terms of multiple poly...

Plasmonic silver nanoparticles have unique properties that lend themselves to unusual optical applications, potentially including use as absorption amplifiers in dye-sensitized solar cells (DSSCs). However, these particles are easily damaged under oxidizing conditions. Atomic layer deposition of TiO2 onto transparent-conductive-oxide-supported silver particles was examined as a means of protect...

Cell transfection is an essential technique for interrogating cellular pathways. Determining the optimal conditions for introducing nucleic acids into cells can be a time consuming endeavor at the initiation of a cell biology experiment or screen. Here we demonstrate how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment. Util...

To understand mechanisms of regulation of dynein activity along and around the axoneme we further characterized the "dynein regulatory complex" (drc). The lack of some axonemal proteins, which together are referred to as drc, causes the suppression of flagellar paralysis of radial spoke and central pair mutants. The drc is also an adapter involved in the ATP-insensitive binding of I2 and I3 inn...