Methods have been developed for the quantitative assay of cytochrome oxidase, esterase, and 11 acid hydrolases in rat-spleen homogenates. These methods seem to be applicable also to other lymphoid tissues. Preliminary studies, extended to nine of the acid hydrolases, indicate that these enzymes occur in partly latent and sedimentable form and that they can be unmasked and rendered soluble by so...

Soil stability includes both resistance, the ability to withstand a perturbation or stress, and resilience, recover pre levels. The functional of soil microbial communities is paramount importance for ecosystem functioning. We investigated differences in (resistance resilience) three enzyme activities (hydrolytic, laccase peroxidase) different forest (holm oak, black pine beech) soils after add...

To determine the influence of variations in both lipid species and lipid packing on phospholipase A2 (PLA2) hydrolytic activity, the activities of two PLA2 isolated from Crotalus molossus molossus venom, were followed on unilamellar liposomes modified by membrane-active peptides. Enzymatic activity was compared with cytolytic activity on human and mouse lymphocytes. Phosphatidylcholine liposome...

Samples of rumen digesta were collected from 2 cows fed with a 100:0 or 40:60 forage/concentrate diet 1 h before and 2 h after feeding. Enzymes from particle-associated microorganisms were extracted by 5 different methods, including physical (freezing, grinding and sonication) or chemical and enzymatic (autolysis in a buffer, lysozyme and CCl4) treatments. Hydrolytic enzyme activities were meas...

In order to improve the activities of mycelium-bound ester hydrolases of Aspergillus oryzae Cs007, the main production conditions were investigated. The activities of the ester hydrolases were simultaneously determined by titration assay and spectrophotometric assay methods, using olive oil and p-nitrophenyl esters as substrates, respectively. The optimum carbon source and nitrogen source were ...

This study is the first comprehensive investigation of enzyme-producing bacteria isolated from four sludge samples (primary, secondary, press and machine) collected in a Kraft paper mill. Overall, 41 strains encompassing 11 different genera were identified by 16S rRNA gene analysis and biochemical testing. Both biodiversity and enzymatic activities were correlated with sludge composition. Press...

Data on the diversity and distribution of enzyme activities in native ectomycorrhizal (ECM) communities are inadequate. A microplate multiple enzymatic test was developed which makes it possible to measure eight enzyme activities on 14 individual, excised ECM root tips. Hydrolytic and oxidative enzymes are involved in the decomposition of lignocellulose, chitin and phosphorus-containing organic...