In this paper we develop data mining techniques to predict 3D contact potentials among protein residues (or amino acids) based on the hierarchical nucleationpropagation model of protein folding. We apply a hybrid approach, using a Hidden Markov Model to extract folding initiation sites, and then apply association mining to discover contact potentials. The new hybrid approach achieves accuracy r...

The BTB domain is a protein–protein interaction motif found throughout eukaryotes. Here we report the identification of two closely related BTB domain-containing proteins, POZ/BTB CONTAINING-PROTEIN 1 (POB1) and POB2, as negative regulators of defense against pathogens. In yeast two-hybrid assays, POB1 and POB2 dimerize through their BTB domains. The pob1–1 pob2–1 double mutant plants exhibited...

When a strain of Escherichia coli, expressing a hybrid protein GalK-beta-Gal, is shifted to high osmolarity, the beta-galactosidase activity strongly decreases within 20 min of shock. The loss of beta-galactosidase activity results from degradation of the hybrid protein under osmotic stress. The results raise the possibility that osmotic stress induces a specific osmodependent protease.

Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approach...

Our group recently prepared a hybrid catalyst containing a rhodium complex, Rh(Cp)(cod), with a maleimide moiety at the peripheral position of the Cp ligand. This compound was then inserted into a β-barrel protein scaffold of a mutant of aponitrobindin (Q96C) via a covalent linkage. The hybrid protein is found to act as a polymerization catalyst and preferentially yields trans-poly(phenylacetyl...

The three-hybrid system can be used to identify RNA sequences that bind a specific protein by screening a hybrid RNA library with a protein-activation domain fusion as 'bait.' These screens complement biochemical techniques, for example, SELEX, co-immunoprecipitation, and cross-linking experiments (see UV crosslinking of interacting RNA and protein in cultured cells and PAR-CLIP (Photoactivatab...