Gliomas are brain-born tumors with devastating impact on their brain microenvironment. Novel approaches employ multiple combinations of chemical compounds in synthetic hybrid molecules to target malignant tumors. Here, we report on the chemical hybridization approach exemplified by artesunic acid (ARTA) and naturally occurring triterpene betulinic acid (BETA). Artemisinin derived semisynthetic ...

We have studied the effect of laminin on type IV of this proteinase activity can also be induced by tumor collagenolytic activity elaborated by malignant cells in promoters such as 12-0-tetradecanoylphorbol 13-acetate (5). culture. Laminin (at concentrations of 4-8 d m l ) It is not known how the production or secretion of this enzyme added to serum-free culture supernatants of subconis cont...

We demonstrate that the genomes of Saccharomyces cerevisiae and S. paradoxus are sufficiently divergent to allow their differential labeling by genomic in situ hybridisation (GISH). The cytological discrimination of the genomes allowed us to study the merging of the two genomes during hybrid mating. GISH revealed that in hybrid nuclei the two genomes are intermixed. In hybrid meiosis, extensive...

Endonuclease restriction (Hind III) fragments of DNA from Chinese hamster X mouse somatic cell hybrids hybridized with proline-rich protein complementary DNA clones only when the DNA was isolated from cells containing mouse chromosome 8, or a fragment of chromosome 8. The evidence suggests that proline-rich protein genes are located at the proximal portion of chromosome 8 toward the centromere.

This study addresses a practical aspect of hybrid dye-sensitized photoelectrochemical cells by exploring simple method to prepare multicomponent systems. Building on previously reported methodo...

Four clones of a somatic monkey-mouse hybrid cell line were studied. One of these clones produced both mouse and monkey interferon, while the three others produced only mouse interferon. All four were, however, sensitive to both mouse and monkey (or human) interferon. The karyotype analysis of these four hybrid clones and the parental cell lines enabled us to locate the possible genetic site go...

Previous studies with a variety of transplantable mouse tumours showed that in hybrids between malignant and non-maJignant cells, malignancy behaved as a recessive character: the hybrid cells, so long as they retained something close to the complete parental chromosome sets, had little or no ability to grow progressively in vivo. In the experiments we now describe the heritable lesions determin...

A wide range of different kinds of malignant cell were fused with certain derivatives of the L cell line and the ability of the resulting hybrid cells to grow progressively in vivo was examined. In all cases the highly malignant character of the tumour cells was suppressed by fusion with the L cell derivatives, whether or not these had metabolic defects that facilitated selection of the hybrid ...

We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually...