The hyaluronic acid-binding region was prepared by trypsin digestion of chondroitin sulfate proteoglycan aggregate from the Swarm rat chondrosarcoma, and biotinylated in the presence of hyaluronic acid and link protein. After isolation by gel filtration and HPLC in 4 M guanidine HCl, the biotinylated hyaluronic acid-binding region was used, in conjunction with avidin-peroxidase, as a specific p...

Previous reports from this laboratory demonstrated the synthesis of hyaluronic acid by a cell-free preparation obtained from a strain of Group A streptococcus. The enzymic activity which sedimented at 105,000 x g, but not at 10,000 x g, required the addition of uridine diphospho-N-acetylglucosamine, uridine diphosphoglucuronic acid, and Mg++ for the formation of hyaluronic acid (l-3). Since hya...

The main object of this paper is to report measurements made on a small series of human synovial fluids and on a few sheep and ox fluids. These measurements were made in order to test the two methods suggested by Ogston & Stanier (1953 a) for detecting differences of the specific properties of the hyaluronic acid as it occurs in synovial fluids. These methods, both of which are based on viscosi...

Introduction: Hearing loss is fairly a common disorder which is usually treated with corticosteroids via systemic administration and/or intra-tympanic injection. This study aimed to compare the effectiveness of intra-tympanic injections of dexamethasone with its combination with hyaluronic acid in patients with sudden sensorineural hearing loss.   Materials and Methods: In this clinical trial, ...

Section on Tissue Biophysics and Biomimetics, NICHD, National Institutes of Health, 13 South Drive, Bethesda, MD 20892, USA E-mail: [email protected] Summary: Aggrecan is a charged biopolymer whose complexes with hyaluronic acid provide the compressive resistance of cartilage. In cartilage extracellular matrix aggrecan-hyaluronic acid complexes are enmeshed within a network of collagen fibr...

Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent & Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 microgram/mg wet wt. to 2.5 micrograms/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased fr...

BACKGROUND Although the safety profile of hyaluronic acid fillers is favorable, adverse reactions can occur. Clinicians and patients can benefit from ongoing guidance on adverse reactions to hyaluronic acid fillers and their management. METHODS A multinational, multidisciplinary group of experts in cosmetic medicine convened the Global Aesthetics Consensus Group to review the properties and c...

Acute pseudoseptic arthritis is a very rare complication that is associated with intra-articular hyaluronic acid injections, which normally involve minimal risk. The most common adverse events that are caused by hyaluronic acid injections are inflammatory reactions or flares at the injection site. In this study, we described three cases of acute pseudoseptic arthritis that was caused by hyaluro...

CD44 is a polymorphic glycoprotein expressed on the surface of many tissues and cell lines which has been implicated in a number of cellular functions including lymphocyte homing to mucosal lymphoid tissue (Peyers patches), leukocyte activation, lymphopoiesis, and tumor metastasis. The predominant isoform found on human leukocytes, CD44H, is a receptor for hyaluronic acid. Because of the promin...

Objective To evaluate the efficacy of intra-articular viscosupplementation therapy with hyaluronic acid for pain relief of knee osteoarthritis, we conducted a meta-analysis of randomized, doubleblinded, placebo-controlled trials. Methods We searched systematically for randomized, double-blinded, placebo-controlled trials of hyaluronic acid (hyaluronan and hylan G-F20) for pain relief of knee os...