interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...

BACKGROUND Metabolic alterations relevant to postprandial dyslipidemia were previously identified in the intestine of obese insulin-resistant subjects. The aim of the study was to identify the genes deregulated by systemic insulin resistance in the intestine of severely obese subjects. METHODS Transcripts from duodenal samples of insulin-sensitive (HOMA-IR < 3, n = 9) and insulin-resistant (H...

OBJECTIVE Humanin (HN), an endogenous antiapoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. We evaluated the effects of a potent analog of HN (HNG) in an in vivo murine model of myocardial ischemia and reperfusion. METHODS AND RESULTS Male C57BL6/J mice (8 to 10 week old) were subjected to 45 minutes of left coronary artery ...

background and objectives: baculovirus can be used as a vector in gene delivery system. viral envelope of baculovirus would display expressed protein/peptide and it could render as a potential vaccine delivery system. in this regard, the gene coding for a subunit of shiga toxin (stxa) from escherichia coli ( e. coli ) strain was cloned in a baculovirus expression system. stxa subunit has the ab...

b ackground: l eishmania is an intracellular parasite infecting humans and many wild and domestic animals. recent studies have suggested an important role for cytotoxic t cells against leishmania . peptide-based vaccines targeting short sequences derived from known immunogenic proteins have been shown to elicit cellular immune responses against disparate pathogens. m ethods: we predicted four h...