Bitter taste receptors (T2Rs) are involved in bitter perception, which is one of the five basic modalities mammals. In this study, a cell co-culture sensor using different proportions Caco-2 cells and SH-SY5Y was proposed. cells, endogenously expressed human T2R38 receptor, T2R16 were co-cultured. Using at constant total concentration 40 K/mL, we designed seven mixtures with [Caco-2]/([Caco-2] ...

Introduction: D-galactose (D-gal) is well known as an appropriate agent to induced aging effects in the in vivo and in vitro models. In the present study, we selected crocin, the main constituent of Crocus sativus L. (Saffron), against D-gal cytotoxicity in human neuroblastoma SH-SY5Y cells. Materials and Methods:  Cells were pretreated with crocin (25-500 µM) for 24 h and then expos...

introduction: d-galactose (d-gal) is well known as an appropriate agent to induced aging effects in the in vivo and in vitro models. in the present study, we selected crocin, the main constituent of crocus sativus l. (saffron), against d-gal cytotoxicity in human neuroblastoma sh-sy5y cells. materials and methods:  cells were pretreated with crocin (25-500 µm) for 24 h and then exposed to d-gal...

All-trans retinoic acid (ATRA) is currently used in adjuvant differentiation-based treatment of residual or relapsed neuroblastoma (NB). It has been reported that short-term ATRA treatment induces migration and invasion of SH-SY5Y via transglutaminase-2 (Tgase-2). However, the detailed mechanism of Tgase-2's involvement in NB cell invasion remains unclear. Therefore we investigated the role of ...

The aim of the present study was to examine the protective effects and mechanism of sika deer (Cervus nippon Temminck) velvet antler polypeptides (VAPs) against MPP+ exposure in the SH‑SY5Y human neuroblastoma cell line. MPP+ cytotoxicity and the protective effects of VAPs on the SH‑SY5Y cells were determined using an MTT assay. Cell apoptosis and mitochondrial membrane potential were detected ...

Enterovirus 71 (EV71) is a pathogenic microorganism that causes hand, foot and mouth disease. However, the epigenetic mechanisms behind how EV71 regulates host cell proliferation and apoptosis are unclear. In the present study, the ability of EV71 to induce apoptosis was analyzed in the SH-SY5Y human neuroblastoma cell line and the effect of this virus on the mRNA expression levels of various a...

The intracellular signaling pathways mediating the neurotrophic actions of pituitary adenylate cyclase-activating polypeptide (PACAP) were investigated in human neuroblastoma SH-SY5Y cells. Previously, we showed that SH-SY5Y cells express the PAC(1) and VIP/PACAP receptor type 2 (VPAC(2)) receptors, and that the robust cAMP production in response to PACAP and vasoactive intestinal peptide (VIP)...

Isoflurane is a commonly used inhaled anesthetic, which induces apoptosis of SH‑SY5Y cells in a dose‑ and time‑dependent manner; however, the underlying mechanisms remain unknown. The authors of the present study hypothesized that a mutation in β‑amyloid precursor protein (APP), which is a gene associated with familial Alzheimer's disease, may render cells vulnerable to isoflurane‑induced cytot...