Previous investigations involving mononuclear cell antibody-dependent cellmediated cytotoxicity (ADCC) toward human red cell (RBC) targets indicated that monocytes but not lymphocytes are able to produce erythrocyte lysis. We reexamined this reported insensitivity of human RBC to lymphocyte cytotoxicity using a variety of human RBC targets and a wide range of antisera to various erythrocyte ant...

To explore the biofunctions of human B7-H3 on activated T lymphocyte, the gene of human B7-H3 encoding the extracellular region (IgV-like and IgC-like domains) was obtained by RT-PCR from human lung cells and subcloned into the prokaryotic expression vector pGEX-5X-3 to express glutathione S-transferase (GST) fusion protein. A 49 kD fusion protein (named as GST/hB7-H3 hereafter) was induced by ...

In order to further understand Epstein-Barr virus (EBV)-lymphocyte interactions, we investigated a chain of events including: (i) EBV binding to human lymphocyte subpopulations; (ii) the earliest appearance of EBV-determined nuclear antigen (EBNA) in the lymphocytes after EBV infection; and (iii) establishment of continuous lymphoblastoid cell lines (LCL) by infecting with EBV different types o...

the production of murine monoclonal antibody (mab) has not yet been reported in iran. the present work describes for the first time the generation of several murine hybridoma clones secreting mabs directed against human leukocyte surface antigens. the secreted antibodies by hybridoma clones have been screened on different lymphoid and non-lymphoid tissues. results indicated that of seven hybrid...

The production of fully immunologically competent humanized mice engrafted with peripheral lymphocyte populations provides a model for in vivo testing of new vaccines, the durability of immunological memory and cancer therapies. This approach is limited, however, by the failure to efficiently engraft human B lymphocytes in immunodeficient mice. We hypothesized that this deficiency was due to th...

human peripheral blood monocytes (hpbm) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. after a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. paralleli...

Authors’ address: V. Kondi and Natalia Mitrica, Centre of Haematology, Bucarest (Rumania) In a previous study, we have shown that sera with anti-platelet antibodies from thrombocytopenic patients or those having received multiple blood transfusions – females alloimmunized by pregnancy – are able to induce inhibition of platelet aggregation [2]. The present investigation is designed to study the...

to extend our knowledge about such interactions substantially beyond that afforded by other presently used methods. In essence, the method uses primary explants of human cancers in autologous and short-term tissue culture systems and permits several useful measurements of distinctive types of lymphocyte activities. In particular, the presence or absence of different types of lymphocyte activiti...