this study was conducted to examine if allergic contact dermatitis (acd) alters the expression of mmps in human dermal fibroblasts. fibroblasts are the primary source for mmp and matrix production in skin. mmps are known to involve in a number of physiological and pathological processes. some published data indicated a gelatinase-like activity in acute and chronic phases of allergic contact der...

The cranial bones and dermis differentiate from mesenchyme beneath the surface ectoderm. Fate selection in cranial mesenchyme requires the canonical Wnt effector molecule β-catenin, but the relative contribution of Wnt ligand sources in this process remains unknown. Here we show Wnt ligands are expressed in cranial surface ectoderm and underlying supraorbital mesenchyme during dermal and osteob...

Catechols were synthesized from safrole. Nine derivatives were prepared and assessed for antiproliferative effects using different human cell lines. The in vitro growth inhibition assay was based on the sulphorhodamine dye to quantify cell viability. The derivatives 4-allylbenzene-1,2-diol (3), 4 4-[3-(acetyloxy)propyl]-1,2-phenylene diacetate (6) and 4-[3-(acetyloxy)propyl]-5-nitro-1,2-phenyle...

Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to determine the effect of extract three medicinal plants, royal jelly, metformin on survival human dermal fibroblast (HDF) cells under oxidative stress induced by H2 O2 . Methods: Hydroalcoholic extracts were prepared from Nigella sativa, Carum carvi seeds, Taraxacum officinale flower. The growth curve HDF...

Stem cells and their nearby niche generate an organ architecture essential to its function. For hair production, the follicle remodels epithelial through stem cell behaviors under instruction of a juxtaposed fibroblast -- dermal papilla. Although papilla’s signaling crosstalk with epithelium is well-characterized, little known about how physical organized support cells. Here, we leveraged live ...

Determinant factors leading from stem cells to megakaryocytes (MKs) and subsequently platelets have yet to be identified. We now report that a combination of nuclear factor erythroid-derived 2 p45 unit (p45NF-E2), Maf G, and Maf K can convert mouse fibroblast 3T3 cells and adult human dermal fibroblasts into MKs. To screen MK-inducing factors, gene expressions were compared between 3T3 cells th...

Loss of gap junctional intercellular communication (GJIC) is a characteristic of cancer cells. Since a coordinated interaction of epithelial tumor cells with stromal cells is a prerequisite for tumor invasion and metastasis, the present study was designed to test the hypothesis that skin-derived tumor cells may modulate homologous and heterologous GJIC. While homologous GJIC of human dermal fib...