نتایج جستجو برای: hsp90 beta

تعداد نتایج: 192746  

Journal: :The Journal of biological chemistry 2002
Giuliano Siligardi Barry Panaretou Philippe Meyer Shradha Singh Derek N Woolfson Peter W Piper Laurence H Pearl Chrisostomos Prodromou

In vivo activation of client proteins by Hsp90 depends on its ATPase-coupled conformational cycle and on interaction with a variety of co-chaperone proteins. For some client proteins the co-chaperone Sti1/Hop/p60 acts as a "scaffold," recruiting Hsp70 and the bound client to Hsp90 early in the cycle and suppressing ATP turnover by Hsp90 during the loading phase. Recruitment of protein kinase cl...

عزیزیان, سارا, مدرسی فر, خشایار, مروج, حمیده, میر معصومی, معصومه, نیک نژاد, حسن,

Background and Objective: It has recently been shown that the application of amniotic membrane conditioned medium is effective in cancer treatment. In this study, the effect of amniotic stem cells conditioned medium on the activity of Hsp90 and Cdk4 expression, were investigated in cancer cells. Materials and Methods: Two cancer cell lines HeLa and MDA-MB-231 were treated with the supernatan...

2010
Daniela Trisciuoglio Chiara Gabellini Marianna Desideri Elio Ziparo Gabriella Zupi Donatella Del Bufalo

BACKGROUND Hypoxia-Inducible Factor 1 (HIF-1) is a transcription factor that is a critical mediator of the cellular response to hypoxia. Enhanced levels of HIF-1alpha, the oxygen-regulated subunit of HIF-1, is often associated with increased tumour angiogenesis, metastasis, therapeutic resistance and poor prognosis. It is in this context that we previously demonstrated that under hypoxia, bcl-2...

Journal: :The Journal of clinical investigation 2015
Klaus-Dieter Preuss Michael Pfreundschuh Martin Weigert Natalie Fadle Evi Regitz Boris Kubuschok

Posttranslationally modified proteins serve as autoimmunogenic targets in a wide spectrum of autoimmune diseases. Here, we identified a posttranslationally modified paraprotein target (paratargs) in monoclonal gammopathies of undetermined significance (MGUS), multiple myelomas (MM), and Waldenstrom's macroglobulinemias (WM) using protein macroarrays that were sumoylated and screened for reactiv...

Journal: :The Journal of biological chemistry 1986
E Nishida S Koyasu H Sakai I Yahara

We have found that the 90-kDa heat shock protein (HSP90) prepared from a mouse lymphoma exists in homodimeric form under physiological conditions and has the ability to bind to F-actin (Koyasu, S., Nishida, E., Kadowaki, T., Matsuzaki, F., Iida, K., Harada, F., Kasuga, M., Sakai, H., and Yahara, I. (1986) Proc. Natl. Acad. Sci. U.S.A., in press). Here we show that calmodulin regulates the bindi...

2016
Mark R. Woodford Diana M. Dunn Adam R. Blanden Dante Capriotti David Loiselle Chrisostomos Prodromou Barry Panaretou Philip F. Hughes Aaron Smith Wendi Ackerman Timothy A. Haystead Stewart N. Loh Dimitra Bourboulia Laura S. Schmidt W. Marston Linehan Gennady Bratslavsky Mehdi Mollapour

Heat shock protein-90 (Hsp90) is an essential molecular chaperone in eukaryotes involved in maintaining the stability and activity of numerous signalling proteins, also known as clients. Hsp90 ATPase activity is essential for its chaperone function and it is regulated by co-chaperones. Here we show that the tumour suppressor FLCN is an Hsp90 client protein and its binding partners FNIP1/FNIP2 f...

2017
Adrienne L. Edkins

Hsp90 is a molecular chaperone that regulates the function of numerous oncogenic transcription factors and signalling intermediates in the cell. Inhibition of Hsp90 is sufficient to induce the proteosomal degradation of many of these proteins, and as such, the Hsp90 chaperone has been regarded as a promising drug target. The appropriate functioning of the Hsp90 chaperone is dependent on its ATP...

Journal: :Molecular cell 2011
Daniel R Southworth David A Agard

Hsp90 is an essential molecular chaperone required for the folding and activation of many hundreds of cellular "client" proteins. The ATP-dependent chaperone cycle involves significant conformational rearrangements of the Hsp90 dimer and interaction with a network of cochaperone proteins. Little is known about the mechanism of client protein binding or how cochaperone interactions modulate Hsp9...

Journal: :Cell 2012
Mikko Taipale Irina Krykbaeva Martina Koeva Can Kayatekin Kenneth D. Westover Georgios I. Karras Susan Lindquist

HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC3...

Journal: :Molecular vision 2007
Alice Banh Paula A Deschamps Mathilakath M Vijayan Jacob G Sivak Judith A West-Mays

PURPOSE This study investigates the effects of heat shock treatment and the role of Hsp70 and Hsp90 on tranforming growth factor beta 2 (TGF-beta2)-induced epithelial-to-mesenchymal transition (EMT) in rat lens epithelial explants. METHODS Rat lens epithelial explants from 7 to 10 day-old Wistar rats were dissected and incubated for 24 h before treatment. The explants were divided into eight ...

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