OBJECTIVES The aim of this study was to develop a simpler and more rapid analytical method for unbound teicoplanin in serum. METHODS A new analytical method was developed by modifying an existing fluorescence polarization immunoassay (FPIA) method. The validation of the developed FPIA method was compared in the quantification of unbound teicoplanin with that of the high-performance liquid chr...

Two rapid assay procedures based on visible spectrophotometry and high performance liquid chromatography (HPLC) have been developed for the determination of amlodipine besylate (ADB) in pharmaceutical formulations. Spectrophotometric method is based on the bromination of ADB with a known excess of bromate-bromide mixture in acid medium followed by the determination of surplus bromine by reactin...

Introduction: To establish the pharmacognostical and phytochemical standardization parameters of Pyrus pashia fruits in order to ensure quality and safety of this traditionally acclaimed medicinal tree. Methods: The fresh fruits of P. pashia were collected and dried. Fruit was subjected to various pharmacognostical investigations, Extraction procedures, and preliminary phytochemical screening, ...

Reversed-phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC) methods have been developed and validated for simultaneous estimation of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms. RP-HPLC separation was achieved on a Phenomenex C18 column using methanol/0.02 mol L-1 ammonium acetate buffer/triethylamine (79.9 + 20 + 0....

BACKGROUND The aim of our work was to develop and validate a reliable LC-MS/MS-based measurement procedure for the quantification of vancomycin in serum, to be applied in the context of efforts to standardize and harmonize therapeutic drug monitoring of this compound using routine assays. METHODS Sample preparation was based on protein precipitation followed by ultrafiltration. In order to mi...

Vitex agnus-castus L., (Verbenaceae) traditional Chinese medicinal plant recommended for various inflammatory ailments, but its mechanism of anti-inflammatory action is still unclear. The present study reports the anti-inflammatory activities of various extracts and elucidate possible mechanism(s) with the quantification of flavonoids by RP-HPLC. The petroleum ether, ethyl acetate, methanol and...

We have developed an on-line solid-phase extraction (SPE) method coupled to high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) for measuring trace levels of 18 perfluorinated chemicals (3 perfluorosulfonates, 8 perfluorocarboxylates, 7 perfluorosulfonamides) in serum. Without protein precipitation, only dilution with 0.1 M formic acid, one aliquot of 100 microL of se...

Competitive reverse-transcription PCR (RT-PCR) techniques for quantification of gene expression employ titrations in which the products of multiple PCRs must be separated, analyzed and quantified to compute gene expression in a single sample. We have employed a novel, ion-pair reversed-phase HPLC (IP-RP-HPLC) system to analyze and quantify RT-PCRs performed with mutant RNA internal standards. P...

The great potential of capillary HPLC (capHPLC) coupled to element mass spectrometry (ICPMS) to study peptide phosphorylation dynamics was evaluated, and the high precision associated with the absolute quantification of the phosphopeptides provided the most detailed phosphorylation time profile ever reported.

BACKGROUND N-Lauroyl-N-methylglucamide is a biodegradable surfactant derived from renewable resources. In an earlier study, we presented an enzymatic solvent-free method for synthesis of this compound. In the present report, the HPLC method developed to follow the reaction between lauric acid/methyl laurate and N-methyl glucamine (MEG) and its environmental assessment are described. RESULTS U...