This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml for CCNU. The recovery ratio of CCNU was 6...

Radioisotope-based and mass spectrometry coupled to chromatographic techniques are the conventional methods for monitoring HMG-CoA reductase (HMGR) activity. Irrespective of offering adequate sensitivity, these methods are often cumbersome and time-consuming, requiring the handling of radiolabeled chemicals or elaborate ad-hoc derivatizing procedures. We propose a rapid and versatile RP-HPLC me...

Measurement of plasma homocysteine may be of value in several clinical conditions including homocystinuria, ath-erosclerosis, thrombophilia, and folate/vitamin B 12 deficiency. The increasing interest in measuring total homo-cysteine in plasma has led to the development of several different methods [1]. A widely used technique for measuring total plasma homocysteine is reversed-phase HPLC with ...

The present study describes the development of a validated RP-HPLC method for the determination of 5-fluorouracil in presence of its degradation products or other pharmaceutical excipients. Stress studies were performed on 5-fluorouracil and it was found that it degrades sufficiently in alkaline conditions, while negligible degradation was observed in acidic, neutral, oxidative and photolytic c...

A fast and selective HPLC method for assaying amphotericin B in biological samples was developed and validated. The chromatographic separation was achieved in less than 12 min on a reverse-phase C(18) column using an acetonitrile-acetic acid-water (52:4.3:43.7, v/v/v) mixture as mobile phase. The flow rate was 1 mL/min and the effluent was monitored at 406 nm. A linear response over the concent...

A sensitive, stability-indicating liquid-chromatographic method for analysis of tamsulosin in the presence of its degradation products has been developed and validated. Efficient chromatographic separation was achieved on a C18 column with a simple isocratic mobile phase consisting methanol: 50 mM phosphate buffer (pH 7.8, ratio 80:20) at a flow rate of 1 ml/m. The linearity of the method was e...

OBJECTIVES Development and validation of a direct enzymatic HbA1c assay that utilizes a single channel on chemistry auto-analyzers without the need to run separate glycated hemoglobin and total hemoglobin assays. DESIGN AND METHODS An enzyme based single channel assay was developed to measure %HbA1c in human whole blood samples. The performance characteristics of the Diazyme Direct Enzymatic ...

Herein we report on a 96-well plate assay based on the fluorescence resulting from the ring-closing metathesis of two profluorophoric substrates. To demonstrate the validity of the approach, four commercially available ruthenium-metathesis catalysts were evaluated in six different solvents. The results from the fluorescent assay agree well with HPLC conversions, validating the usefulness of the...

Purpose: To develop and validate a sensitive and specific HPLC assay for cladribine (CdA) in plasma for pharmacokinetic studies in rats. Methods: CdA and the internal standard AZT were purchased from Sigma-Aldrich Chem. The HPLC system consisted of a Shimadzu LC-9A pump, a 3 μm, 250 x 2.0 mm I.D. high speed C18 column (Jupiter), preceded by a 5 μm 4 x 4 mm I.D. C18 guard column (Licrocart), an ...