Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select Hy-resistant (HyR) Ustilago maydis transformants. This is an example of an Ascomycete promoter functioning in a Basidiomycete. HyR transformants varied with respect to copy number of integr...

allometric growth pattern and body shape changes of the green terror (andinoacara rivulatus)(cichlidae) were studied using landmark-based geometric morphometric (gm) and traditional methods, from hatching up to 1266 hours post hatching (hph) under culture conditions. the left side of specimens were photographed using digital camera and morphometric characters, including total length, head lengt...

Dictyostelium discoideum has proven an exceptionally powerful system for studying numerous aspects of cellular and developmental functions. The relatively small ( approximately 34 Mb) chromosomal genome of Dictyostelium and high efficiency of targeted gene disruption have enabled researchers to characterize many specific gene functions. However, the number of selectable markers in Dictyostelium...

Background: The objective of this study was to develop an efficient selectable marker for transgenic Dunaliella salina. Results: Tests of the sensitivity of D. salina to the antibiotic chloramphenicol and the herbicide Basta® showed that cells (1.0 × 10 cells/ml) treated with 1000 or 1500 μg/ml chloramphenicol died in 8 or 6 days, respectively, whereas D. salina cells (1.0 × 10 cells/ml) treate...

BACKGROUND Genetic manipulation of poxvirus genomes through attenuation, or insertion of therapeutic genes has led to a number of vector candidates for the treatment of a variety of human diseases. The development of recombinant poxviruses often involves the genomic insertion of a selectable marker for purification and selection purposes. The use of marker genes however inevitably results in a ...

in this paper, a feasibility study of building a human powered hydrofoil (hph) vessel is reported. hydrofoil vessels are a well-known class of high-speed crafts. in addition to high-speed operation, the hydrofoils have a reliable maneuvering capability, good stability and proper operation in waves. also, a human powered vehicle, nowadays is an advancing idea. different aspects of the design and...

Allelic replacement in the Burkholderia genus has been problematic due to the lack of appropriate counter-selectable and selectable markers. The counter-selectable marker sacB, commonly used in gram-negative bacteria, is nonselective on sucrose in many Burkholderia species. In addition, the use of antibiotic resistance markers of clinical importance for the selection of desirable genetic traits...

The current study addresses the influence of pectin depletion on potential high-pressure homogenization (HPH) to increase network forming cell wall material (CWM) from tomato. Therefore, microstructure, water binding capacity and viscoelastic properties alcohol-insoluble residue (AIR) unextractable fractions (UFs) obtained after different levels methods extraction were determined in suspension ...

BACKGROUND Antibiotic/ herbicide resistant marker genes have been proven to be very useful in plant transformation for the initial selection of desired transgenic events. However, presence of these genes in the genetically modified crops may render the crop less acceptable to the consumers. Among several different approaches, the effectiveness of Cre/lox mediated recombination strategy for sele...