First posted May 27, 2021 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 Between June 2018 and July 2019, the U.S. Survey collaborated with State Department of Environmental Conservation to analyze water quality in seven embayments on Long Island, York, for a study examine fecal contamination using microbial s...

Unexpected meeting of two separate lines of research resulted in the discovery of oncogenes. Oncogenes are deoxyribonucleic acid (DNA) sequences coding for polypeptide gene products which cause, or contribute to, neoplastic growth of cells. Oncogenes remain almost unchanged through evolution: oncogenes and their gene products of avian, murine, feline, simian and human species show close homolog...

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort i...

Since the invention of the polymerase chain reaction (PCR), >26 000 papers have been published that in some way utilizethis technique for DNA analysis. Clinical applications remain largely in the research laboratory setting, but the first US Food and Drug Administrationapproved PCR-based diagnostic kit for Chiamydia trachomatis is already commercially available, and the PCR technique holds grea...

Previous results have shown that infection of KB cells with type 5 adenovirus elicits the inhibition of host protein synthesis. In an attempt to determine whether inhibition is caused by a block in the transcription of host deoxyribonucleic acid (DNA), the rate of synthesis of host DNA-like ribonucleic acid (D-RNA) was compared with the rate of host protein synthesis at various times after infe...

background: nowadays, highly specific aptamers generated by cell selex technology (systematic evolution of ligands by exponential enrichment) are being applied for early detection of cancer cells. prostate specific membrane antigen (psma), over expressed in prostate cancer, is a highly specific marker and therefore can be used for diagnosis of the prostate cancer cells. the aim of the present s...

With the birth of next-generation sequencing (NGS) technology, genomic data in public databases have increased exponentially. Unfortunately, exogenous contamination or intracellular parasite sequences assemblies could confuse analysis. Meanwhile, they can provide a valuable resource for studies host-microbe interactions. Here, we used strategy based on DNA barcodes to scan protistan GenBank WGS...

A series of experiments was conducted to study the base composition of DNA in AVl2-induced tumor and host cells by paper chromatography, and it was found that DNA per cent. guaninecystosine contents were around 42 % in both of them. The base composition of DNA of AV12 itself differs considerably from that of AVl2-induced tumor cells, while the DNA of tumor cells shows the property similar to th...

Murine leukemia viruses (MLVs) are among the simplest retroviruses. Prototypical gammaretroviruses encode only the three polyproteins that will be used in the assembly of progeny virus particles. These are the Gag polyprotein, which is the structural protein of a retrovirus particle, the Pol protein, comprising the three retroviral enzymes-protease, which catalyzes the maturation of the particl...

We describe a new PCR-based method for distinguishing human and cow fecal contamination in coastal waters without culturing indicator organisms, and we show that the method can be used to track bacterial marker sequences in complex environments. We identified two human-specific genetic markers and five cow-specific genetic markers in fecal samples by amplifying 16S ribosomal DNA (rDNA) fragment...