BACKGROUND Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of R...

A compound AgGa2Te3I belongs to the quaternary semiconductors of general formula AІВІІІ2X3Y (AІ – Cu, Ag; ВІІІ Ga, In; X S, Se, Те; Y - Cl, Br, I). For first time, its structure, which forms in tetragonal syngony, was studied by powder method, Sp.Gr. I-4, structural type CuIn2Te3Cl, with cell parameters: a = 6,0041 (4) Å, c 11,965 (1) Å. The synthesized evacuated quartz ampoules from simple sub...

instead of (1). Clearly, (3) is a homogeneous LP and is self-dual which essentially follows from the constraints form a skew-symmetric system. The interpretation of (3) is τ is a homogenizing variable and the constraints represent primal feasibility, dual feasibility, and reversed weak duality. The homogeneous model (3) was first studied by Goldman and Tucker [2] in 1956 and they proved (3) alw...

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

Large scale disturbances are known to impact the alpha and beta diversity of communities. However, whether these increase or decrease is often debated. The goal this study was quantify how seedling community impacted within across elevation in El Yunque forest Puerto Rico following a major hurricane. We tested two alternative hypotheses, that hurricanes relatively more homogenizing non-homogeni...

Law school training has a certain centralizing, homogenizing, tendency . . . Centrism pulls to the center, not because the center has any particular content, but because, well . . . it is the center . . . [I]n the American law schools a particular school of jurisprudence has already won out. The triumph of this school of thought is so complete, so pervasive, that it is not even seen as a school...

A particle-free extract has been prepared from rat liver which is capable of incorporating isotopic acetate, pyruvate, and acetyl CoAl into long chain fatty acids. While both cytoplasmic and mitochondrial enzymes are necessary for the complete system, evidence is presented suggesting that the presence of electrolyt,es in the homogenizing medium may act to extract the lipogenic enzymes of the mi...