traumatic brain injury (tbi) is physical damage to the brain structure which has a high global rate of mortality and morbidity. tbi can cause intense inflammatory response due to accumulation of leukocytes in cerebral matrix and activation of microglia. microglia can differentiate into m1 macrophages or m2 macrophages following the changes in biochemical properties of brain tissue. m1 sub type ...

Biophysical properties of the cellular microenvironment, including stiffness and geometry, have been shown to influence cell function. Recent findings implicated 3D confinement as an important regulator behavior. The understanding how mechanical signals direct function is based primarily on 2D studies. To investigate extent affects function, a single culture platform fabricated with geometrical...

A new natural halogen-containing stilbene derivative was isolated from the leaves of Cajanus cajan (L.) Millsp. and identified as 3-O-(3-chloro-2-hydroxyl-propanyl)-longistylin A by comprehensive spectroscopic and chemical analysis, and named cajanstilbene H (1). It is the first halogen-containing stilbene derivative found from plants. In human mesenchymal stem cells (hMSC) from bone marrow, 1 ...

BACKGROUND After intramyocardial injection, mesenchymal stem cells (MSCs) may engraft and influence host myocardium. However, engraftment rate and pattern of distribution are difficult to control in vivo, hampering assessment of potential adverse effects. In this study, the role of the engraftment patterns of MSCs on arrhythmicity in controllable in vitro models is investigated. METHODS AND R...

Long-term cultures of telomerase-transduced adult human mesenchymal stem cells (hMSC) may evolve spontaneous genetic changes leading to tumorigenicity in immunodeficient mice (e.g., hMSC-TERT20). We wished to clarify whether this unusual phenotype reflected a rare but dominant subpopulation or if the stem cell origin allowed most cells to behave as cancer stem cells. Cultures of the hMSC-TERT20...

Twenty-eight (8 ruminally cannulated) lactating, multiparous Holstein cows were blocked by DIM and randomly assigned to 7 replicated 4 x 4 Latin squares (28-d periods) to investigate the effects of different dietary ratios of alfalfa silage (AS) to corn silage (CS) on production, N utilization, apparent digestibility, and ruminal metabolism. The 4 diets contained (dry matter basis): A) 51% AS, ...

Split thickness skin graft (STSG) implantation is one of the standard therapies for full thickness wound repair when full thickness autologous skin grafts (FTG) or skin flap transplants are inapplicable. Combined transplantation of STSG with dermal substitute could enhance its therapeutic effects but the results remain unsatisfactory due to insufficient blood supply at early stages, which cause...

This work considers implementation of requirements expressed as High-level Message Sequence Charts (HMSCs). All HMSCs are not implementable, but a particular subclass called local HMSCs can be implemented using a simple projection operation. This paper proposes a new technique to transform an arbitrary HMSC specification into a local HMSC, hence allowing implementation. We show that this transf...

Abstract INTRODUCTION Brain metastases are the most common brain tumors and occur in 10-30% of cancer patients, whereas leptomeningeal disease (LMD) occurs approximately 5% adults with systemic malignancies. Tractable preclinical models that faithfully represent metastasis to recapitulate LMD needed improve our understanding biological basis CNS as well developing effective therapeutic strategi...

Methods hMSC cell culture. Human mesenchymal stem cells (hMSCs) were obtained from Lonza Corporation (Wakersville, MD). For encapsulation studies, hMSCs were expanded in growth media (α-MEM, 10% FBS, 1% L-Glutamine & penicillin-streptomycin) and encapsulated at passage 3 in MeHA, MeAlg, MeDex (all at 15 x 10 cells mL) or MeMaHA (10 cells mL) hydrogels. For studies evaluating hMSC differentiatio...