A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

The Nidānakathā as it is preserved in the Pāli Canon, and in the Tibetan bKa’ ’gyur, has come to us via canonical traditions and an extensive network of commentarial traditions that began in India and were continued later in Ceylon. These commentarial traditions began at a very early period, possibly from the time of the death of the Buddha, and were inspired by the need to establish certain fa...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Several important issues are pertinent to an ethical discussion of the new technology today grouped under the name Assisted Reproduction Technology (ART). This is a moral imperative because each of us must decide what is to be considered ethically acceptable in assisted reproduction. Reflecting on the ethical dimension of ‘creating’ a new human life can play a major role in ensuring that in eac...

Because of the immensity of Augustine’s corpus and the complex intellectual patrimony that informs it, attempts to place him within the history of philosophical traditions are often partial and in need of supplementation. In treating below of Augustine’s engagement with Aristotelianism, Middle Platonism, Neoplatonism, and Stoicism, I shall be drawing attention to particular topics, lexical poin...