Radioactive leucine was injected into the cavai vein of normal and Morris hepatoma 9121-bearing rats. Livers and hepatomas were removed 12 min later. Within this time, no newly synthesized radioactive protein had left the liver, since labeled protein appeared in the blood no earlier than 15 min after injection. Albumin was purified from livers and hepatomas to constant specific radioactivity. T...

about 6 times increased over that with host liver in both systems, and incorporation with hepatoma 7777 is about 10 times that with host liver in both the in vivo and in vitro systems. Addition of sucrose to the incubation mixture (0.8 M final concentration) increases incorporation in host liver nuclei twoto threefold but has no effect on hepatoma nuclei. This difference in response to a more d...

MicroRNA (miRNA or miR) inhibition of oncogenic related pathways has been shown to be a promising therapeutic approach for cancer. SIRT1 might be a promoter factor on tumorigenesis of hepatocellular carcinoma (HCC). However, the mechanism is unknown. We investigated whether miRNAs regulate the SIRT1 and its downstream SREBP-lipogenesis-cholesterogenesis metabolic pathway in hepatoma cells. Huma...

Radioactive leucine was injected into the cavai vein of normal and Morris hepatoma 9121-bearing rats. Livers and hepatomas were removed 12 min later. Within this time, no newly synthesized radioactive protein had left the liver, since labeled protein appeared in the blood no earlier than 15 min after injection. Albumin was purified from livers and hepatomas to constant specific radioactivity. T...

The starch gel electrophoretic isozyme patterns of phosphoglucomutase. glucose-6-phosphate dehydrogenase, hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase in the cancerous and in the apparently uninvolved regions of livers from human primary hepatoma patients were compared. The HK, PK, and lactate dehydrogenase isozyme patterns in hepatoma were also compared with those in normal...

BACKGROUND Homeobox B7 (HOXB7) has been identified associated with poor prognosis of hepatocellular carcinoma (HCC). However, the specific mechanism by which HOXB7 promotes the malignant progression of HCC remains to be determined. METHODS Immunohistochemistry (IHC) was used to detect the expression level of HOXB7 in 77-paired HCC tissue samples, and the correlation between HOXB7 and HCC prog...

The extents of anabolism and catabolism in vitro of purines and purine ribonucleotides by rat tissues, Morris Hepatoma 5123, and Reuber H-35 hepatoma were deter mined. The results are compared with those obtained previously for Novikoff hepato ma. Hepatoma 5123 was more active catabolically than any of the host tissues exam ined, including liver; H-35 tumor was about one-half as active cataboli...

Sodium cyanate at a dose level of 125 or 250 mg/kg i.p. caused an inhibition of incorporation of 3H-labeled amino acids into cytoplasmic and nuclear proteins of the rapidly growing hepatoma 7777 and the slowly growing hepatoma 9618A. There was no inhibitory effect on 3H-labeled amino acid incorporation into protein in the livers of rats bearing these tumors. Studies on the effects of sodium cya...

The effect of apple polyphenol extract (APE) on the proliferation and invasion of a rat ascites hepatoma cell line of AH109A was examined in vitro. APE suppressed both the hepatoma proliferation and invasion in a dose-dependent manner up to 200 mug/ml. Serum obtained from rats orally given APE also inhibited hepatoma proliferation and invasion when added to the culture medium. Subsequently, the...

This paper describes a quantitative procedure for the isolation of 67 Ga-binding granules (GBG) from normal rat liver and Morris 5123C hepatoma homogenates by a combination of rate and isopyknic density gradient zonal centrifugation. Another class of GBG has been found that is much smaller than the lysosomal GBG we have previously described. These smaller particles, or microvesicles, bind the l...