Maternal consumption of a high-fat diet (HFD) during pregnancy and lactation is closely related to hepatic lipid accumulation, insulin resistance and increased serum cytokine levels in offspring and into their adulthood. MicroRNA (miRNA) have been implicated in cholesterol biosynthesis and fatty acid metabolism. We evaluated the modulation of hepatic fatty acid synthesis (de novo), β-oxidation ...

The concentrations of malonyl-CoA, citrate, ketone bodies and long-chain acylcarnitine were measured in freeze-clamped liver samples from fed or starved normal, partially hepatectomized or sham-operated rats. These parameters were used in conjunction with measurements of the concentration of plasma non-esterified fatty acids and the rates of hepatic lipogenesis to obtain correlations between ra...

Background: Utilization of energy-dense lipid fuels is critical to the rapid development and growth of neonates. Objective: To increase efficiency of milk fat utilization by newborn pigs, the effect of clofibrate on in vivo and in vitro longchain fatty acid (LCFA) oxidation was evaluated. Methods: Newborn male pigs were administered 5 mL of vehicle (2% Tween 80) with or without clofibrate (75 m...

BIIbmdARY t a r r 7 a'j';QI 7 1`he peroxidative system involving hepatic catalase plays a major role in t e dation of 6 i c el a /^ ` i methanol in the rat (1), but in the monkey the peroxidative mechanism does not appear to ^0, J be important. This conclusion is based on the following observations: (a) ethanol and °r y methanol were about equally reactive with the peroxidative system, but etha...

Rats with long-term cholestasis have reduced ketosis during starvation. Because it is unclear whether this is also the case in short-term cholestasis, we investigated hepatic fatty acid metabolism in rats with bile duct ligation for 5 days (BDL5, n = 11) or 10 days (BDL10, n = 11) and compared the findings with those made with pair-fed control rats (CON5 and CON10, n = 11). The plasma beta-hydr...

BACKGROUND Utilization of energy-dense lipid fuels is critical to the rapid development and growth of neonates. OBJECTIVE To increase efficiency of milk fat utilization by newborn pigs, the effect of clofibrate on in vivo and in vitro long-chain fatty acid (LCFA) oxidation was evaluated. METHODS Newborn male pigs were administered 5 mL of vehicle (2% Tween 80) with or without clofibrate (75...

1. The aim of this study was to determine the effect of a constant infusion of glucose on the ketosis that is observed when dairy cows are deprived of food in early lactation. 2. Cows in early lactation were first deprived of food for 4 days (96h) to induce a 'fasting ketosis'. Glucose was then infused intravenously at a constant rate of 0.75 g/min for 48h while deprivation of food was maintain...

T HE ENZYME, XANTHINE OXIDASE, is thought to play a central role in the mobilization of storage iron from the liver.1 In the presence of xanthine oxidase, xanthine is oxidized to uric acid, the enzyme acting as electron acceptor in the oxidation reaction . ‘ Coupled to the oxidation of uric acid is the reduction of ferric ferritin to the ferrous state. The livers of immature rats contain very l...

Liver-specific phosphoenolpyruvate carboxykinase (PEPCK) null mice, when fasted, maintain normal whole body glucose kinetics but develop dramatic hepatic steatosis. To identify the abnormalities of hepatic energy generation that lead to steatosis during fasting, we studied metabolic fluxes in livers lacking hepatic cytosolic PEPCK by NMR using 2H and 13C tracers. After a 4-h fast, glucose produ...

Nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disease in humans, is characterized by the accumulation of triacylglycerols (TGs) in hepatocytes. We tested whether 2',3',5'-tri-acetyl-N6-(3-hydroxylaniline) adenosine (IMM-H007) can eliminate hepatic steatosis in hamsters fed a high-fat diet (HFD), as a model of NAFLD. Compared with HFD-only controls, IMM-H007 treatment s...