نتایج جستجو برای: hen egg white lysozyme
تعداد نتایج: 230888 فیلتر نتایج به سال:
Herein we report a new strategy for protein refolding by taking advantage of the unique surface and pore characteristics of ethylene-bridged periodic mesoporous organosilica (PMO), which can effectively entrap unfolded proteins and assist refolding by controlled release into the refolding buffer. Hen egg white lysozyme was used as a model protein to demonstrate the new method of protein refoldi...
We have employed dynamic light scattering and X-ray small-angle scattering for monitoring the cluster formation of hen-egg white lysozyme solutions under crystallization conditions. From time-resolved dynamic light scattering experiments we can obtain a set of observables that exhibit clear extrema, when simultaneously plotted as functions of protein and electrolyte molarity. Close to these ext...
Crystallization conditions for hen egg white lysozyme in the presence of various ions were determined at pH 4.5 and 18 degrees C. The corresponding solubility curves show that the main effects are due to anions in the following order: SCN- greater than NO3- greater than Cl- greater than citrate2- greater than CH3COO- approximately H2PO4- greater than SO4(2-). This is in the reverse order of the...
The onset of hen egg white lysozyme aggregation on exposure to alkaline pH of 12.2 and subsequent slow growth of soluble lysozyme aggregates (at 298 K) was directly monitored by steady-state and time-resolved fluorescence anisotropy of covalently attached dansyl probe over a period of 24 h. The rotational correlation time accounting for tumbling of lysozyme in solution (40 microM) increased fro...
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