Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most co...

background : as prenatal screening for sexually transmitted infections and treatment of infected pregnant women is not routinely performed in iran and prevalence of two sexually transmitted pathogens, chlamydia trachomatis and mycoplasma genitalium , in sabzevar (east of iran) is unknown, we decided to perform this prospective study. methods : one hundred ninety-six urine specimens of pregnant ...

haemoplasmas contain eperythrozoon and haemobartonella species, which are widespread causes of animal and human infections. these obligatory cell parasites were previously known as hemobartonella; now hemotropic mycoplasmas. these bacteria are identified by the lack of cell wall and their small genome. the usual diameter is between 0.3 and1μm. mycoplasmasarepolymorphic and seen in circular or b...

We report here the draft genome sequences of Mycoplasma alkalescens, Mycoplasma arginini, and Mycoplasma bovigenitalium. These three species are regularly isolated from bovine clinical specimens, although their role in disease is unclear.

Haemoplasmas contain Eperythrozoon and Haemobartonella species, which are widespread causes of animal and human infections. These obligatory cell parasites were previously known as Hemobartonella; now hemotropic mycoplasmas. These bacteria are identified by the lack of cell wall and their small genome. The usual diameter is between 0.3 and1μm. Mycoplasmasarepolymorphi...

Haemoplasmas contain Eperythrozoon and Haemobartonella species, which are widespread causes of animal and human infections. These obligatory cell parasites were previously known as Hemobartonella; now hemotropic mycoplasmas. These bacteria are identified by the lack of cell wall and their small genome. The usual diameter is between 0.3 and1μm. Mycoplasmasarepolymorphi...

We read with great interest the data reported by Castilla and Wadowsky about the elimination of a Mycoplasma hominis-like mycoplasma from the TW-183 strain of Chlamydia pneumoniae and its lack of effect on chlamydial infection of HEp-2 cells (1). We recently described detection of Mycoplasma fermentans, M. hominis, and M. hyorhinis in several strains of C. trachomatis and C. pneumoniae, the suc...

Agalactia is an infectious and contagious disease of small ruminants caused by Mycoplasma agalactiae (M. agalactiae). Although different microorganism strains contribute to this disease,  M. agalactiae is known as the most prominent causative agent. Therefore, this study aimed to investigate the rate of M. agalactiae involvement in contagious agalactia in the southeast region of Iran. Sampling ...

Mycoplasmas cause several diseases in cattle as pneumonia in calves and mastitis in cows and also reproductive disorders, abortions, arthritis and conjunctivitis. The difficulty of culturing mycoplasmas in vitro is a major obstacle to research and laboratory diagnosis of these fastidious organisms. A total of 120 preputial swabs, 70 vaginal swabs and 65 milk samples were collected from cattle a...

We describe the development and analytical validation of a 7-plex polymerase chain reaction assay coupled to a bead-based liquid suspension array for detection of multiple ruminant Mycoplasma spp. The assay employs a combination of newly designed and previously validated primer-probe sets that target genetic loci specific for Mycoplasma bovis, Mycoplasma mycoides cluster, Mycoplasma mycoides su...